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Molecular cloning of murine decay accelerating factor by immunoscreening |
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| Authors: | Fukuoka, Yoshihiro Yasui, Akira Okada, Noriko Okada, Hidechika |
| Affiliation: | 1 Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University Seiryo-machi, Sendai 980-77, Japan 2 Department of Neuroscience Institute of Development, Aging and Cancer, Tohoku University Seiryo-machi, Sendai 980-77, Japan 3 Department of Molecular Biology, Nagoya City University School of Medicine Mizuho-cho, Nagoya 467, Japan |
| Abstract: | Although the cDNA of human decay accelerating factor (DAF) whichrestricts complement activation on homologous cell membraneswas cloned in 1987, all trials to detect the cDNA of mouse DAFby cross-hybridization were unsuccessful. However, by immunoscreeningwith a rabbit antiserum against purified mouse DAF, we successfullycloned the cDNA. It contains four typical short consensus repeats(SCR) similar to that In human and guinea pig DAF. The basesequence showed 63.7 and 63.8% identIty to that of human andguinea pig DAF respectively. The deduced amino acid sequenceidentity to human and guinea pig DAF was 47.2 and 46.5% respectively.Mouse complement receptor related gene Y (Crry)/p65 functionis comparable to DAF. SCR3 and SCR4 of mouse DAF showed 50%identity to SCR2 and SCR3 of Crty/p65 respectively. IdentificatIonof the mouse DAF gene should open a new approach for determiningthe actual in vlvo role of DAF by analyzing autoimmune miceas well as generating DAF gene knockout mice using embryonicstem cells. |
| Keywords: | decay accelerating factor glycosylphosphatidylinositol anchor short consensus repeat |
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