Enzymatic determination of total cholesterol in serum by flow injection analysis

Several methods are proposed for the enzymatic determination of total cholesterol in serum by flow injection analysis (FIA). The use of the merging-zones mode, with or without halting of the reacting plug in the photometric or fluorimetric detector, has allowed the development of four precise methods for the determination of this analyte; calibration curves were linear for ranges of 0.02-0.2 and 0.005-0.05 gl(-1). These methods have been applied to the determination of cholesterol in serum with excellent results.