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板蓝根空间诱变初步效应
引用本文:陈向东,兰进,王晓光. 板蓝根空间诱变初步效应[J]. 中药材, 2007, 30(4): 381-383
作者姓名:陈向东  兰进  王晓光
作者单位:中国医学科学院中国协和医科大学药用植物研究所,北京,100094
摘    要:为探明飞船搭载板蓝根发生的变异,辅助选育高产优质新品种。本试验采用薄层色谱法鉴别,在精氨酸溶液相应的位置上,搭载与未搭载样品之间都显相同颜色的斑点;同工酶电泳结果显示样品之间的酯酶同工酶酶带存在差异,而过氧化物同工酶除了酶染色程度有所不同外,在酶带数目上没有变化。折干率、浸出物含量的测定结果显示样品之间存在差异。初步研究结果表明搭载板蓝根发生了遗传变异,但性状不够稳定,有必要做进一步研究。

关 键 词:板蓝根  飞船搭载  同工酶  折干率  浸出物
文章编号:1001-4454(2007)04-0381-03
修稿时间:2006-07-18

Primary Effects on Isatis indigotica after Spaceflight
CHEN Xiang-dong,LAN Jin,WANG Xiao-guang. Primary Effects on Isatis indigotica after Spaceflight[J]. Journal of Chinese medicinal materials, 2007, 30(4): 381-383
Authors:CHEN Xiang-dong  LAN Jin  WANG Xiao-guang
Affiliation:Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China
Abstract:Isatis indigotica carried by the Chinese first spaceship "Shenzhou" was studied in order to find the mutation after spaceflight.TLC differentiation experiments showed no distinct discrepancy among the samples of spaceflight and non-space-flight,and the same color spot appeared corresponding to the location of the arginine.Isoenzymes of esterase and peroxidase were studied with PAGE.Isoenzymes of esterase were difference among the samples.To peroxidases,little difference was found with them.The ratio of dry weight and extract contents showed out the mutation has emerged after spaceflight,but some characters were unstable.It is necessary for further study.
Keywords:Isatis indigotica  Spaceflight  Isoenzymes  Dry weight  Extract contents
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