| 顺铂预处理化疗通过免疫调节增强CIK细胞对B16恶性黑色素瘤的抑制作用 |
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| 引用本文： | 陈景,黄香,宋海珠,陈龙邦.顺铂预处理化疗通过免疫调节增强CIK细胞对B16恶性黑色素瘤的抑制作用[J].癌症进展,2011,9(6):631-638,645. |
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| 作者姓名： | 陈景 黄香 宋海珠 陈龙邦 |
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| 作者单位： | 南京大学医学院临床学院南京军区南京总医院肿瘤内科,南京,210002;南京大学医学院临床学院南京军区南京总医院肿瘤内科,南京,210002;南京大学医学院临床学院南京军区南京总医院肿瘤内科,南京,210002;南京大学医学院临床学院南京军区南京总医院肿瘤内科,南京,210002 |
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| 基金项目： | 国家自然科学基金项目（No30872979）; 南京军区医学科研课题（09MA080） |
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| 摘 要： | 目的 观察顺铂( Cisplatin,DDP)预处理化疗联合CIK细胞对B16恶性黑色素瘤的抑制作用,探讨DDP预处理化疗增强细胞因子诱导的杀伤细胞(cytokine - induced killer cells,CIK cells)抑瘤作用的潜在机制.方法 建立C57BL/6小鼠B16黑色素瘤模型,测量肿瘤体积,绘制...
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| 关 键 词： | 预处理化疗 细胞因子诱导的杀伤细胞 调节性T细胞 树突状细胞 髓系来源抑制细胞 |
Cisplatin enhances the antitumor activity of cytokine -induced killer cells through modulation of immune cells |
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| Chen Jing,Huang Xiang,SongHaizhu,Chen Longbang.Cisplatin enhances the antitumor activity of cytokine -induced killer cells through modulation of immune cells[J].Oncology Progress,2011,9(6):631-638,645. |
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| Authors: | Chen Jing Huang Xiang SongHaizhu Chen Longbang |
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| Institution: | Chen Jing Huang Xiang SongHaizhu ChenLongbang Department of Medical Oncology,Nanjing General Hospital of Nanjing military Command,Medical School of Nanjing University,Nanjing 210002,China |
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| Abstract: | Objective To observe the tumor inhibition of DDP preconditioning combined with cytokine-induced killer cells（CIKs） in mice-bearing B16 melanoma model and to elucidate the underlying mechanisms DDP precondition for depressing tumor growth of CIKs.Methods Murine melanoma models were made and tumor size was measured as indicators of therapeutic response.The numbers of Treg infiltrating in tumor tissue were detected through immunohistochemical analysis.The percentages of dendritic cells（DC）,myeloid-derived suppressor cells（MDSC） and CIK cells in various tissues were analyzed through flow cytometry.Results The combination of DDP preconditioning chemotherapy and CIK cells significantly inhibited the growth of B16 melanoma（P0.05）.Pretreatment with DDP decreased the Treg in the local tumor tissues.DDP facilitated CIK cells homing to tumor tissues and TDLN.DDP up-regulated the percentages of DC in bone marrows,peripheral bloods and spleen tissues and diminish the levels of MDSC in bone marrows,peripheral bloods,spleen tissues TDLN.Conclusion DDP preconditioning chemotherapy can enhance the antitumor activity of CIK cell therapy in B16 melanoma model through modulation of endogenous and exogenous immune cells in various tissues. |
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| Keywords: | Preconditioning chemotherapy cytokine-induced killer cells regulatory T cells dendritic cells myeloid-derived suppressor cells |
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