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SEPT4蛋白在特发性弱精子症患者精子中的表达
引用本文:Li YS,Feng XX,Ji XF,Wang QX,Gao XM,Yang XF,Pan ZH,Sun L,Ma K. SEPT4蛋白在特发性弱精子症患者精子中的表达[J]. 中华男科学杂志, 2011, 17(8): 699-702
作者姓名:Li YS  Feng XX  Ji XF  Wang QX  Gao XM  Yang XF  Pan ZH  Sun L  Ma K
作者单位:郑州大学第三附属医院人类精子库,河南,郑州,450052
基金项目:郑州市科技攻关计划项目(112PPTSF318-7)~~
摘    要:目的:探讨SEPT4蛋白在特发性弱精子症发生机制中的作用。方法:采用非连续密度梯度离心分离和纯化精子,免疫细胞化学技术检测SEPT4在特发性弱精子症患者和正常男性精子中的定位及表达变化,同时用RT-PCR和W estern印迹技术,从基因和蛋白水平检测SEPT4的表达及差异。结果:免疫细胞化学表明,SEPT4定位于精子环并且在特发性弱精子症患者精子中的表达显著低于正常男性(94.75±19.95vs111.51±17.57,t=3.452,P<0.01);RT-PCR显示,特发性弱精子症患者精子中SEPT4 mRNA的表达水平与正常男性相比显著降低(0.56±0.15vs0.71±0.16,t=3.521,P<0.05);W estern印迹结果与RT-PCR结果一致,SEPT4蛋白在特发性弱精子症患者精子中的表达亦显著低于正常男性(0.48±0.20vs0.77±0.18,t=5.872,P<0.05)。结论:SEPT4在特发性弱精子症患者精子中的表达水平显著降低,可能是导致弱精子症发生的原因之一。

关 键 词:SEPT4蛋白  特发性弱精子症  精子环  逆转录-聚合酶链反应  免疫印迹

Expression of SEPT4 protein in the ejaculated sperm of idiopathic asthenozoospermic men
Li Yu-Shan,Feng Xiao-Xia,Ji Xiao-Fei,Wang Quan-Xian,Gao Xue-Min,Yang Xian-Feng,Pan Zhou-Hui,Sun Lin,Ma Kui. Expression of SEPT4 protein in the ejaculated sperm of idiopathic asthenozoospermic men[J]. National journal of andrology, 2011, 17(8): 699-702
Authors:Li Yu-Shan  Feng Xiao-Xia  Ji Xiao-Fei  Wang Quan-Xian  Gao Xue-Min  Yang Xian-Feng  Pan Zhou-Hui  Sun Lin  Ma Kui
Affiliation:LI Yu-shan,FENG Xiao-xia,JI Xiao-fei,WANG Quan-xian,GAO Xue-min,YANG Xian-feng,PAN Zhou-hui,SUN Lin,MA KuiHuman Sperm Bank,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450052,China
Abstract:Objective: To investigate the role of the SEPT4 protein in the pathogenesis of idiopathic asthenozoospermia.Methods: Samples of ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men were separated and purified by Percoll discontinuous density gradients,the distribution and expression of SEPT4 in the sperm samples were determined by immunocytochemistry,and the expressions of SEPT4 mRNA and SEPT4 protein were detected by RT-PCR and Western blot.Results: Immunocytochemistry showed...
Keywords:SEPT4 protein  idiopathic asthenozoospermia  annulus  RT-PCR  Western blot  
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