Increased surface expression of class I MHC molecules on immunogenic cells derived from the xenogenization of P815 mastocytoma cells with 8-methoxypsoralen and long-wavelength ultraviolet radiation

Abstract: In a previous study we demonstrated that the treatment of the highly tumorigenic cell line, P815, with 8-methoxypsoralen and long-wave-length ultraviolet radiation resulted in the production of several immunogenic clones (tum—^-). Mice inoculated with the tum^- cells survived much longer than mice inoculated with the original tumorigenic cells (tum⁺). It was suggested that the increased survival of mice treated with the tum^- clones arose as a result of an increased antigenicity derived from the phototreatment. In this report we show that the tum^- cells have a greater density of class I MHC molecules on their surface (50–157% compared to P815). Class I MHC density on the cell surface is required to elicit targeted cytotoxic responses. These results can be considered in terms of human class I MHC assays which show that many human tumor cells have a reduced expression of class I MHC. Because other DNA damaging agents have also been shown to enhance class I expression, it is suggested that in addition to the cytotoxic effects of these agents, other pleiotropic effects must be considered. Photochemotherapy may phenotypically alter cells so that the enhanced expression of class I MHC molecules on the surface of phototreated cells may be associated with the clinical responses observed in cutaneous T cell lymphoma patients.