不同地域阴道毛滴虫分离株的随机扩增多态DNA技术分析

目的　分析比较阴道毛滴虫 7个分离株基因组DNA的遗传多态性。　方法　应用随机扩增多态DNA技术对阴道毛滴虫北京 1株、北京 2株、承德株、唐山株、九江 1株、九江 2株与九江 3株基因组扩增 ,并对扩增产物进行聚类分析。　结果　 7虫株两两间的遗传相似指数为 77.4%～ 94.7% ,遗传关系密切。其中 :北京 1株和唐山株为 89.2 % ,九江 1株和九江 2株为 92 .1% ,北京 2株和九江 3株为 94.7% ,亲缘关系较近。而九江 1株和承德株间遗传相似指数为77.4% ,同源性相对较低。　结论　 7株阴道毛滴虫遗传关系密切 ,但在基因水平上存在种内差异性 ;地理位置对阴道毛滴虫遗传学特性影响不大。

RAPD Analysis on Different Isolates of Trichomonas vaginalis

Objective To study genetic polymorphism of DNA on seven isolates of Trichomonas vaginalis. Methods The random amplified polymorphic DNA (RAPD) technique was performed to amplify genomic DNA of the seven T. vaginalis isolates, including Beijing 1, Beijing 2, Chengde, Tangshan, Jiujiang 1, Jiujiang 2 and Jiujiang 3. The DNA bands detected were analyzed by clustering analysis with SPSS software. Results The percentage of genetic similarity among the seven isolates was from 77.4% to (94.7%,) showing a close genetic relationship among them. The percentages between the isolates of Beijing 1 and Tangshan, Jiujiang 1 and Jiujiang 2, Beijing 2 and Jiujiang 3 were 89.2%, 92.1% and 94.7% respectively, while that of Jiujiang1 and Chengde was 77.4%, indicating a lower homology. Conclusion There are a close genetic relationship and certain gene polymorphism among the seven T. vaginalis isolates; geographical origin plays little role to the genetic characteristics.