Characterization of inflammatory reaction induced by neuwiedase, a P-I metalloproteinase isolated from Bothrops neuwiedi venom |
| |
Authors: | Daiana Silva Lopes,Carolina de Freitas Oliveira,Jaqueline D. Dias Oliveira,Amé lia Hamaguchi,Ana Maria Moura-da-Silva,Veridiana de Melo Rodrigues |
| |
Affiliation: | a Laboratório de Imunopatologia, Instituto Butantan, Av. Dr. Vital Brasil 1500, 05503-900 São Paulo, SP, Brazil b Laboratório de Química de Proteínas e Produtos Naturais, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil c Departamento de Clínica Médica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil d Laboratório de Biotecnologia, Universidade Federal do Tocantins, Tocantins, Brazil e Laboratório de Genética Molecular, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil |
| |
Abstract: | The Snake Venom Metalloproteinases (SVMPs) play a relevant role in the multifactorial inflammatory response induced by Bothrops envenomations. Neuwiedase, an SVMP isolated from Bothrops neuwiedi venom, is devoid of hemorrhagic activity on skin tests, but is able to induce myonecrosis and degrade fibrinogen, fibrin, type I collagen, fibronectin and laminin. In this study, we analyzed the inflammatory reaction induced by neuwiedase in gastrocnemius muscle, with special focus on cytokines release. Our results showed clear evidence of inflammatory infiltrate in the gastrocnemius muscle and an increase of MMP-9, and the cytokines KC, IL-1β and IL-6 in the early periods after toxin injection. The cytokine release was also evaluated in inflammatory and muscular cell culture. Both murine peritoneal adherent cells (MPACs) and muscle cells (C2C12) released pro-inflammatory cytokines after stimulus with neuwiedase. MPACs showed increased production of KC, IL-1β and IL-6 in the cell culture supernatant while in C2C12, the predominant chemokine expressed was KC. These data reinforce the importance of SVMPs in the inflammatory response caused by envenomation and point out the role of muscle cells in this event by releasing pro-inflammatory mediators able to attract leukocytes to the muscle, thus starting and amplifying the setting of the inflammatory reaction. |
| |
Keywords: | Snake venom Metalloproteinases Neuwiedase Muscle cell, inflammation Cytokines |
本文献已被 ScienceDirect 等数据库收录! |
|