大孔螯合树脂可用于处理中药重金属污染

目的:利用大孔螯合树脂建立一种新的中药重金属污染的处理方法.方法:先对中药粗提物进行乳化和调节pH值至7,将其分别过3种大孔树脂(D401,D402,D001)柱,进而测定处理前后的中药粗提物中的5种重金属(Cu,Pb,Cd,Hg,As)和有效成分类黄酮的含量.结果:经D401和D402处理后,中药粗提物中的重金属含量显著降低,Cu的含量由0.500 mg·L-1降为0.117 mg·L-1和0.236 mg·L-1,Pb的含量由0.521 mg·L-1降为0.174 mg·L-1和0.165 mg·L-1,Cd的含量由0.078 mg·L-1降为0.024 mg·L-1和0.043 mg·L-1,Hg和As的含量分别由0.005 mg·L-1和0.002 mg·L-1降至检测限以下;但经过D001处理后,重金属含量的下降不明显.另外经过3种树脂处理后,类黄酮的量变化很小.我们还发现常温时,当中药提取物的流速为30 m/h和pH为7时,D401的处理效果最好.结论:大孔螯合树脂D401和D402可以用于中药粗提物中超标重金属的处理.

Application of macroporous chelating resins in treatment of heavy metals pollution in traditional Chinese medicine

AIM: To find new methods for heavy metals pollution in traditional Chinese medicine(TCM) with macroporous chelating resins. METHODS: We emulsificated crude extracts of TCM and adjusted its pH to 7 first and the pretreated crude extracts was treated by three kinds of macroporous resins(D401,D402 and D001) respectively,then we detected the concentrations of 5 heavy metals(Cu,Pb,Cd,Hg,As) and flavonoids in the crude extracts with or without treatment. RESULTS: The concentrations of all heavy metals decreased significantly after treatment of D401 and D402.The concentrations(mg/L) of Cu,Pb,Cd changed from 0.500,0.521,0.078 to 0.117 and 0.236,0.174 and 0.165,0.024 and 0.045,respectively,and Hg and As became beyond the detection limit after the treatment,but the use of D001 affected a bit quantities of 5 heavy metals.Moreover,the concentration of flavonoids kept almost the same after treatment of such 3 resins.On the condition of room temperature,we found that a fluent velocity of 30 m/s and pH of 7 would bring the superior treatment of D401 to excessive heavy metals in TCM. CONCLUSION: Macroporous chelating resins(D401 and D402) can be employed in the treatment of excessive heavy metals in crude extracts of TCM.