重庆市8家三级医院血液透析用水和透析液细菌学横断面调查

目的了解重庆市医院透析室透析用水、透析液的内毒素及细菌污染情况。方法采集该市8家三级甲等医院透析用水及透析液标本,进行细菌培养和菌落计数;用ATi动态试管仪测定反渗水及透析液中内毒素含量。结果 8家医院透析用水细菌培养计数均≤200 CFU/mL,1家医院透析液细菌计数2 000 CFU/mL,培养出的细菌以革兰阴性菌为主;透析用水内毒素均2 EU/mL。结论目前重庆市大部分医院血液透析室的透析用水符合国家标准,但仍有部分医院透析液存在细菌污染。应定期检测透析用水、透析液中的内毒素和进行细菌培养,保障医疗安全。

A cross sectional study on bacterial monitoring of reverse osmosis water and dialysate from 8 hemodialysis units in Chongqing

Objective To evaluate the endotoxin and bacterial contamination in reverse osmosis （RO） water and dialysate in hospitals in Chongqing. Methods Specimens of RO water and dialysate were obtained from 8 hemodialysis units in Chongqing;Bacterial culture was performed and endotoxin was measured With ATi dynamic test tube meter. Results Bacterial counts of RO water in 8 hospitals were all ≤200 CFU/mL, bacteria counts of dialysate of 1 blood purification centers were〉2 000 CFU/mL, the majority of isolated bacteria were gram-negative bacteria; the contents of endotoxin in the RO water of 8 centers were all 〈2 EU/mL. Conclusion RO water and dialysate in most hospitals in Chongqin are conformed to national standard, but bacterial contamination of RO water and dialysate is existing in some Chongqing blood purification centers. The regular detection of endotoxin and bacteria should be undertaken to ensure the safety of medical treatment.