二氧化氯消毒剂杀菌效果的实验观察

目的：通过实验室试验研究二氧化氯消毒剂杀灭枯草杆菌黑色变种芽孢、金黄色葡萄球菌和大肠杆菌的最低有效剂量。方法：依据《消毒技术规范》（2002年版）采用悬液定量试验法进行了实验室观察。结果：在3％和0．3％牛血清白蛋白条件下，二氧化氯含量分别为15.2mg／L，作用1min和10min对大肠杆菌平均杀灭对数值均〉5．00；在3％和0．3％牛血清白蛋白条件下，二氧化氯含量分别为20、6mg／L，作用1min对金黄色葡萄球菌平均杀灭对数值均〉5．00；在3％和o．3％牛血清白蛋白条件下，二氧化氯含量分别为100、60mg／L，作用5min对枯草杆菌黑色变种芽孢平均杀灭对数值均〉5．00。结论：实验室的结果略低于生产企业申报检测时的二氧化氯消毒剂作用浓度，在不同实验室有效剂量也有差异，可能由于二氧化氯生产原料和工艺不同所致，最终应以实验数据为准。

Experimental observation on germicidal efficacy of chlorine dioxide disinfectant

Objective:To find the minimum effective olose of chlorine clioxide disinfectant to kill Bacillus subtilis vav nigev spore,Staphylococcus aureus and Escherichia coli.Methods:According to those for evaluating the germicidal efficacy of disinfectants(2002 edition),suspension quantitative germicidal test methods were used to carry out laboratory observation.Results:When the bacterical suspention containing bovine serum albumin 3% and 0.3% was used for test,the killing logartithm of spores of Escherichia coli exposed to the solution containing chlorine dioxide 15 mg/L and 2mg/L for 1 min and 10 min was >5.00,the killing logartithm of spores of Staphylococcus aureus exposed to the solution containing chlorine dioxide 20 mg/L and 6mg/L for 1 min was >5.00,the killing logartithm of spores of Bacillus subtilis vav nigev spore exposed to the soluthow containing chlorine dioxide 100 mg/L and 60 mg/L for 5 min was >5.00.Conclusion:In the laboratory study,the mininum effetive dose of chlorine dioxide disinfectant is slighely less than the corporaton dcclared dose.In the different laboratory,the effective dose is difference.