应用微矩阵表达谱基因芯片检测食管癌的基因改变

目的：应用微矩阵表达谱基因芯片研究食管癌的基因变化。方法：按一步法抽提三例食管癌及其对照癌旁组织的总RNA，分别逆转录成荧光分子掺入的探针，混合后杂交基因芯片（14114种基因）。经严格洗片后用扫描仪扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。结果：在所检测的3例临床标本中，共有的差异表达基因31条，上调基因10条，下调基因21条，有多类基因，有与信号转导有关、与细胞周期调控有关、与癌基因同源的基因等等。结论：食管癌和对照癌旁组织间存在差异表达的基因。

Study on differentially expressed genes in esophageal carcer by cDNA microarray

Objective To screen the differentially expressed genes between esophageal carcer and carcer adjacent tissue using cDNA microarray.Methods The total RNAs were isolated from the tissues,reversely transcribed to cDNAs to prepare the hybridizaion probes.The mixed probes were hybridized to the cDNA microarray(14?114 target genes).After washing,the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues.Results 31 genes whose expression level differe in 3 cases between the tissues,10 genes up regulation,21 genes down regulation.Conclusion the differentially expressed genes between 2 different kinds of tissue.