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广西地区猪、鼠、狗戊型肝炎病毒感染血清学分析
引用本文:韦献飞,梁靖瑞,唐荣兰,葛宪民,熊云新,吉彦莉,杨剑萍,谢益安,朱永红.广西地区猪、鼠、狗戊型肝炎病毒感染血清学分析[J].中国公共卫生,2007,23(2):228-229.
作者姓名:韦献飞  梁靖瑞  唐荣兰  葛宪民  熊云新  吉彦莉  杨剑萍  谢益安  朱永红
作者单位:1. 柳州医学高等专科学校公共卫生系,柳州,545006
2. 北京大学医学部病原生物学系
3. 广西职业病研究所
基金项目:国家自然科学基金;广西卫生厅科研项目
摘    要:目的了解广西地区猪、鼠和狗血清中抗.戊型肝炎病毒(HEV)IgG抗体流行情况。方法应用HEV基因1型开放读码框(ORF)2和ORF3多肽包被酶标板,辣根过氧化物酶(HRP)标记的羊抗猪、羊抗鼠和羊抗狗建立的酶联免疫吸附试验(ELISA)法检测猪、鼠和狗血清中抗.HEV IgG。结果总抗.HEV IgG阳性率为31.84%(170/534),其中猪血清抗.HEV IgG阳性率为26.40%(66/250),鼠血清抗.HEV IgG阳性率为43.02%(77/179),狗血清抗.HEV IgG阳性率为25.71%(27/105)。结论猪、鼠和狗血清中存在HEV感染,鼠抗.HEV IgG阳性率最高。

关 键 词:戊型肝炎病毒  抗-HEV  IgG  动物宿主
文章编号:1001-0580(2007)02-0228-02
修稿时间:06 24 2006 12:00AM

Serum epidemiological survey of hepatitis E virus infection among swine, rats and dogs in Guangxi
WEI Xian-fei, LIANG Jing-rui, TANG Rong-lan. et al..Serum epidemiological survey of hepatitis E virus infection among swine, rats and dogs in Guangxi[J].Chinese Journal of Public Health,2007,23(2):228-229.
Authors:WEI Xian-fei  LIANG Jing-rui  TANG Rong-lan
Institution:Department of Public Health, Liuzhou Medical College Liuzhou 545006. China
Abstract:Objective To investigate hepatitis E virus(HEV) infection in swine,rats and dogs in Liuzhou of Guangxi.Methods Anti-HEV IgG was detected in sera of swine,rats and dogs by an enzyme-linked immunoabsorbent assay(ELISA),which was developed using the synthetic peptides derived from genotype 1 HEV ORF2 and ORF3 sequences.Results The total anti-HEV IgG positive rate was 31.84% (170/534) from all animals tested.Anti-HEV IgG positive rates of swine,rats and dogs were 26.40%(66/250),43.02%(77/179) and 25.71%(27/105) respectively.Conclusion Swine,rats and dogs could be infected by HEV,and the prevalence rate of anti-HEV IgG was the highest in rats.
Keywords:hepatitis E virus  anti-HEV IgG  animal reservoir
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