联合低温保护剂对脐血造血干细胞长期低温保存的作用

目的 观察联合低温保护剂对脐血造血干细胞长期低温保存的作用。方法 60份由联合低温保护剂（5％DMSO＋6％HES＋5％人体白蛋白）保存在岭南脐血库的脐血，按时间分为1，2，3，4年等4组，复温后观察其细胞活力、总有核细胞数（TNC）、CD34^ ,CD34^ CD38^-,CFU-GMey BFU-E的回收率。结果 60例脐血冻存后的细胞活力为96．39％，TNC，CD34^ ,CD34^ CD38^-,CFU-GM及BFU－E的回收率分别是95．89％，94．45％，88．88％,87．77%及83．18％，不同时间组的上述指标之间差异无显著性（P＞0．05）。结论 联合低温保护剂具有效果可靠、操作简单等优点，适用于脐血造血干细胞的长期低温保存。

The effect of combined cryoprotectant on long term cryopreservation of umbilical blood stem cell

Objective To observe the protection of combined cryoprotectant (5%DMSO+6%HES+5%human albumin) on umbilical blood stem cells stored for different cryopreservation duration. Methods 60 cryopreserved umbilical bloods were divided into 4 groups according to duration(1, 2, 3 and 4 years). The viability of cells, total nucleate cells (TNC),CD 34 +, CD 34 +CD 38 -,CFU-GM, and BFU-E were tested before and after cryopreservation with combined cryoprotectant (5%DMSO+6%HES+5%human albumin) . Results The viability of cells,TNC,CD 34 +, CD 34 +CD 38 -,CFU-GM, and BFU-E in 60 cryopreserved umbilical bloods were 96.39%, 95.89%, 94 45%, 88 88%, 87 77% and 83 18% No significant difference were found among different cryopreservation duration groups( P >0 05). Conclusion With the advantages of reliability and simplicity, combined cryoprotectant is desirable long term cryopreservation of umbilical cord blood stem cells