TK基因在垂体腺瘤治疗中的作用

目的探讨胸苷激酶（TK）基因在垂体腺瘤治疗中的作用及其机制。方法应用免疫印迹法，检测AdGHTK质粒转染GH3细胞后TK基因的表达情况;使用非放射性细胞增殖检测法，测定不同感染复数（MOI）AdGHTK转染后的GH3细胞对更昔洛韦的敏感性；将转染AdGHTK的GH3细胞和未转染细胞以不同比例混合，观察旁观者效应。结果TK基因可转染入GH3细胞并成功表达；即使在低MOI下，转染组细胞成活率也随更昔洛韦浓度的增加而降低；存在旁观者效应。结论将TK基因导入GH3细胞并经更昔洛韦处理，可明显降低肿瘤细胞存活率，为垂体腺瘤的治疗提供了可靠的理论依据。

THE EFFECT OF TK GENE ON PITUITARY ADENOMA

Objective To explore the efficacy of thymidine kinase （TK） gene for pituitary adenoma. Methods Applying mmunoblotting, the expression of TK gene was detected after AdGHTK plasmid transfect GH3 cells; and the sensitivity of CH3 cells was determined by non-radioactive cell Proliferation assay, which had been transfected by AdGHTK with different multiplicities of infections （MOls） to the ganciclovir; GH3 cells transfected with AdGHTK were mixed with the non-transfected cells in different ratios to observe the bystander effect. Results TK gene could be transfected into GH3 cells and expressed successfully; even in the condition of lower MOl, the survival rate of cells in transfection group kept decreasing as the concentration of ganciclovir increased. In addition, there existed a bystander effect. Conclusion Transfection of TK gene into GH3 cells and the treatment with GCV obviously reduced the survival rate of tumor cells, which provides a reliable theory basis for the treatment of pituitary adenoma.