| 一种简化的分离与培养小鼠原代肝细胞的方法 |
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| 引用本文： | 张丁丁,辛永宁,罗兵,姜曼,刘昊刚,宣世英. 一种简化的分离与培养小鼠原代肝细胞的方法[J]. 青岛大学医学院学报, 2014, 0(1): 34-36 |
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| 作者姓名： | 张丁丁 辛永宁 罗兵 姜曼 刘昊刚 宣世英 |
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| 作者单位： | [1]青岛大学医学院附属青岛市市立医院消化内二科,山东青岛266011 [2]中国海洋大学医药学院 ,山东青岛266011 [3]青岛大学医学院微生物学教研室 ,山东青岛266011 [4]青岛市消化疾病重点实验室,山东青岛266011 |
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| 基金项目： | 国家自然科学基金资助项目（81170337/H0304） |
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| 摘 要： | 目的 建立一种简单的能获得高纯度小鼠原代肝细胞的分离与培养方法。方法 将经典的两步灌流法简化为经下腔静脉逆向脉冲式灌注,利用低速离心法对肝细胞分离液进行纯化;利用锥虫蓝染色法鉴定细胞活率;培养瓶预先用鼠尾胶原铺备,利用倒置显微镜观察细胞形态。结果 此法得到了足够数量的肝细胞,细胞活率大于90%;细胞可稳定贴壁1周。结论 成功建立了一种简单的分离与培养小鼠原代肝细胞的方法。
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| 关 键 词： | 肝细胞 细胞培养技术 小鼠 |
A SIMPLIFIED METHOD TO ISOLATE AND CULTIVATE PRIMARY MOUSE HEPATIC CELLS |
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| ZHANG Dingding,XIN Yongning,LUO Bing,J IANG Man,LIU Haogang,XUAN Shiying. A SIMPLIFIED METHOD TO ISOLATE AND CULTIVATE PRIMARY MOUSE HEPATIC CELLS[J]. Acta Academiae Medicinae Qingdao Universitatis, 2014, 0(1): 34-36 |
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| Authors: | ZHANG Dingding XIN Yongning LUO Bing J IANG Man LIU Haogang XUAN Shiying |
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| Affiliation: | (Department of Digestive System, The Affiliated Municipal Hospital of Qingdao University Medical College, Qingdao 266011, China) |
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| Abstract: | Objective To create a simple method of isolating and culturing high-purity mouse primary hepatic cells. Methods The classic two-step perfusion technique was simplified to intermittent retrograde perfusion from the inferior vena cava. The cell suspension was purified by a low speed centrifugation. The motility rate was assessed by trypan blue staining. The culture flask was pre-paving by mouse tail collagen. The morphous of the hepatocytes was observed by an invert microscope. Results Adequate hepatocytes were obtained. The motility rate of the hepatocytes was higher than 90%. The viability of the cells could last for one week. Conclusion A simple method of isolation and primary culture of mouse hepatocytes has been successfully created. |
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| Keywords: | hepatocytes cell culture techniques mice |
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