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| 淋巴细胞表面HLA-I类抗原的阻断 | |
| 引用本文： | 张印则,李伟,周华友,夏荣,兰炯采,章扬培,张志欣.淋巴细胞表面HLA-I类抗原的阻断[J].中国实验血液学杂志,2003,11(6):622-624. |
| 作者姓名： | 张印则 李伟 周华友 夏荣 兰炯采 章扬培 张志欣 |
| 作者单位： | 1. 第一军医大学南方医院输血科,广州,510515 2. 北京红十字血液中心,北京,100088 3. 军事医学科学院野战输血研究所,北京,100850 |
| 基金项目： | 国家高技术发展规划863基金资助项目 编号2 0 0 1AA2 61161 |
| 摘 要： | 为了研究阻断淋巴细胞表面HIA—Ⅰ类抗原与其相应抗体特异性结合的方法及效果，在22℃和pH7．4PBS介质中采用甲氧基聚乙二醇-苯并三唑碳酸盐(mPEG—BTC)(12mmol／L)处理淋巴细胞。结果表明，经mPEG—BTC处理后的淋巴细胞与相应HLA-Ⅰ类抗体的微量淋巴细胞毒试验为阴性。结论：在22℃和pH7．4条件下mPEG—BTC可以阻断淋巴细胞表面HIA—Ⅰ抗原与其相应抗体的特异性结合。 |
| 关 键 词： | 淋巴细胞 甲氧基聚乙二醇-苯并三唑碳酸盐 HLA-Ⅰ类抗原 mPEG-BTC 基因 |
| 文章编号： | 1009-2137(2003)06-0622-03 |
| 修稿时间： | 2003年2月27日 |
Camouflage of HLA-I Antigen in Lymphocyte Surface |
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| ZHANG Yin Ze,LI Wei ,ZHOU Hua You,XIA Rong,LAN Jong Cai,ZHANG Yang Pei ,ZHANG Zhi Xin.Camouflage of HLA-I Antigen in Lymphocyte Surface[J].Journal of Experimental Hematology,2003,11(6):622-624. | |
| Authors: | ZHANG Yin Ze LI Wei ZHOU Hua You XIA Rong LAN Jong Cai ZHANG Yang Pei ZHANG Zhi Xin |
| Institution: | Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. zyz200157@sohu.com |
| Abstract: | The objective of this study was to investigate the method and effect of blocking the specific reaction between lymphocyte HLA-I antigen and its antibody. The lymphocytes were disposed with 12 mmol/L methoxypolyethelene glycol benzotriazol carbonate (mPEG-BTC) in concentration gradient in PBS (pH 7.4) at 22 degrees C. The effect of the modified lymphocytes was detected by microlymphocytotoxicity assay. The results showed that lymphocytes modified by mPEG-BTC did not react with related HLA-I antibodies in microcytotoxicity test. It is suggested that the specific reaction between HLA-I antigen of lymphocyte and HLA-I antibodies can be completely camouflaged by mPEG-BTC in PBS (pH 7.4) under 22 degrees C room temperature. |
| Keywords: | lymphocyte mPEG BTC HLA I antigen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |