半叶马尾藻多糖对抑癌基因p53和Rb的影响

目的 研究半叶马尾藻多糖(SP)对抑癌基因p53和R6的影响.方法 称重法检测SP对小鼠S180肉瘤的抑瘤率;RT-PCR分析抑癌基因p53和Rb中mRNA的含量:Western-blotting检测p53和Rb中蛋白质的表达水平.结果 将浓度分别为50、100和200mg/kg的SP经腹腔注射人S180肉瘤小鼠体内,连续10d,抑瘤率分别为30.5%、47.6%和63.5%.SP可上调抑癌基因p53和R6中mRNA的表达以及增加p53和Rb中蛋白质含量.结论 SP对小鼠S180肉瘤具有一定的体内抑制作用,并上调抑癌基因的表达.

Effects of Sargassum confusum polysaccharide on the expression of p53 and Rb genes in mouse sarcoma S180 cells

OBJECTIVE: To investigate effects of Sargassum confusum polysaccharide (SP) on the expressions of p53 and Rb genes. METHODS: The mice bearing S180 sarcoma were treated with abdominal SP injection at the daily dose of 50, 100 or 200 mg/kg for 10 consecutive days, The mice injected with 0.85% saline served as the negative control group and those with 5-flurouracil (5-FU) injection as the positive control group. The tumor weight was measured to calculate the tumor inhibition rate. RT-PCR and Western blotting were used to detect the expression levels of p53 and Rb genes at the mRNA and protein levels, respectively. RESULTS: The 10-day SP treatment at 50, 100 and 200 mg/kg resulted in tumor growth inhibition rates of 30.5%, 47.6% and 63.5%, respectively. SP treatment upregulated the mRNA expressions and increased the protein levels of p53 and Rb in the tumors. CONCLUSION: SP has inhibitory effect against S180 sarcoma in vivo and can increase the expression levels of p53 and Rb genes at both the mRNA and protein levels.