异常黑胆质成熟剂及清除剂对氧化应激诱导的淋巴细胞损伤及基因表达影响的比较研究

目的:以H2O2诱导淋巴细胞的氧化应激模型,观察研究异常黑胆质成熟剂及清除剂对活性氧诱导的细胞损伤和Bcl-2蛋白表达的作用效应的差异。方法:用"彗星实验"(SCGE)和MTT法观察异常黑胆质成熟剂及清除剂对氧化应激的淋巴细胞DNA损伤和细胞生长活性的影响;并采用western-blot研究淋巴细胞Bcl-2蛋白的表达。结果:异常黑胆质成熟剂对氧化应激诱导的淋巴细胞DNA和细胞损伤有保护性作用,而清除剂的作用明显不同;异常黑胆质成熟剂能上调Bcl-2蛋白的表达,而清除剂能下调该蛋白的表达,二者的作用相反。结论:异常黑胆质成熟剂及清除剂对氧化应激的淋巴细胞损伤以及细胞凋亡相关基因表达的作用明显不同。

The Influence of Abnormal Savda Munziq and Mushil on Oxidative Stress Induced Damage and Bcl-2 Expression in Lymphocyte

Objective:To study the effect of Abnormal Savda Munziq and Mushil on H_2O_2-induced lymphocyte DNA and cell damage,and Bcl-2 protein expression.Method:The DNA damage of lymphocytes was detected by Comet Assay and after treatment of Abnormal Savda Munziq and Mushil on H_2O_2 induced lymphocyte cultures.Western blot was employed to determine the expression of Bcl-2 proteins in different treatment lymphocyte groups.Results:Abnormal Savda Munziq has protective effect against H_2O_2-induced DNA damage.By comparison the activity and toxicity of Abnormal Savada Munziq with Mushil,these two medicine are obviously different.The Abnormal Savda Munziq also shows obviously up-regulation upon lymphocyte Bcl-2 protein expression.Contrast to Abnormal Savda Munziq,the Abnormal Savada Munshil shows the opposite effect that can inhibit the lymphocyte Bcl-2 protein expression.Conclusion:The effects of Abnormal Savda Munziq and Mushil the Bcl-2 protein expression and apoptosis induced by oxidative stress in lymphocyte are distinct.