汉防己甲素阻抑大鼠肝纤维化的实验研究

目的：研究汉防己甲素对实验性肝纤维化的作用。方法：用CCl4制备大鼠肝纤维化模型，以鳖甲作为对照。观察汉防己甲素对大鼠肝纤维化各项指标的影响。结果：组织学和生化分析表明，汉防己甲素能减少胶原在肝内沉积；免疫荧光半定量分析提示汉防己甲素组肝内Ⅰ型胶原含量较对照组显著减少；电镜观察发现汉防己甲素组大鼠肝内有些纤维母细胞胞浆中出现髓鞘样结构及自噬体。结论：汉防己甲素具有阻抑肝纤维化的作用，其作用机制可能与该药对纤维母细胞的致细胞病变作用有关。

A Study of Inhibitory Effect of Tetrandrine on Experimental Hepatic Fibrosis

Objective: To study the effect of Tetrandrine on experimental hepatic fibrosis. Methods: Experimental hepatic fibrosis was induced in rats by administration of Carbon Tetrachlorid with Carapax Trionycis as control. The following parameters of hepatic fibrosis were determined. Results: The results indicated that the deposition of collagen in the liver was decreased by administration of Tetrandrine using both histological and biochemical analysis. The hepatic type I collagen content in Tetrandrine group was significantly lower as compared with control group by semiquantitative analysis using immune fluorescent assay. Both myelin figure and autophagosome were found in some cytoplasm of hepatic fibroblasts in Tetrandrine group by electronmicroscope. Conclusion: These finding suggest that Tetrandrine has inhibitory effect on hepatic fibrosis and that the mechanism of this effect may be related to the cytopathogenic action of Tetrandrine on the fibroblast.