热带灵芝的化学成分研究

目的研究热带灵芝(Ganoderma tropicum(Jungh.)Bres.)子实体的化学成分。方法采用常压硅胶柱色谱、减压硅胶柱色谱、Sephadex LH-20柱色谱等分离方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果从热带灵芝的乙醇提取物中分离得到10个化合物,分别鉴定为:3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid methyl ester(1)、灵芝酸I(2)、甲基灵芝酸B(3)、灵芝酮B(4)、灵芝酮A(5)、灵芝酸H(6)、甲基灵芝酸H(7)、24R-ergosta-7,22-diene-3,5,6-triol(8)、22E,24R-5α,6α-epoxyergos-ta-8,22-diene-3β,7α-diol(9)、麦角甾-7,22-二烯-3β-醇(10)。结论化合物1～7为三萜类化合物,化合物8～10为甾体类化合物,均为首次从热带灵芝中分离得到。

Study on the chemical constituents from Ganoderma tropicum ZHAO You-xing2*

＂ Lingzhi＂ is Chinese generic name for basiocarps of Ganoderma, which was widely distributed and well-studied. Ganoderma genera is used widely as a health supplement and ＂herbal＂ medicine. Ganoderma tropicum（ Jungh. ） Bres. is the main wild Ganoderma resources distributed in tropical area of China,and its natural products and their biological activities are still not reported. In this paper, we reported the isolation, structural elucidation of natural compounds from the fruiting bodies of G. tropicum for the first time. During our investigation on G. tropicum, ten compounds were isolated. Their structures were identified as 3β,7β-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid methyl ester （ 1 ）, ganoderenic acid I （ 2 ）, methyl ganoderate B （3）, lucidone B （ 4 ）, lucidone A （ 5 ）, ganoderenic acid H （ 6 ）, methyl ganoderate H （7） ,24R-ergosta-7,22-diene-3,5,6-triol（8） ,22E,24R-5α,6α-epoxyergosta-8,22-diene-3β,7α-diol （9） ,ergosta-7,22-dien-3β-ol （10） by NMR spectra as well as comparison with those reported in the literatures.