| 脉动流场中离体血管段长度与内皮细胞内皮素代谢相关--内皮细胞膜张应力累加效应实验研究 Ⅲ |
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| 引用本文： | 王贵学,胥成浩,蔡绍皙,冯强,王远亮. 脉动流场中离体血管段长度与内皮细胞内皮素代谢相关--内皮细胞膜张应力累加效应实验研究 Ⅲ[J]. 生物医学工程学杂志, 2002, 19(4): 606-610 |
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| 作者姓名： | 王贵学 胥成浩 蔡绍皙 冯强 王远亮 |
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| 作者单位： | 1. 重庆大学,生物工程学院,国家教育部生物力学与组织工程重点实验室,重庆,400044
2. 重庆大学,生物工程学院,国家教育部生物力学与组织工程重点实验室,重庆,400044;乐山师范学院,生物系,乐山,614004 |
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| 基金项目： | 国家自然科学基金(19732003-2)、教育部重点实验室基金项目 |
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| 摘 要： | 用离体血管灌流实验验证冯元桢等关于血管内皮细胞的膜张应力逆血流方向累加的理论分析.长度分别为11、21 cm的离体血管段内皮细胞分别置于平均切应力均为0.12 N/m2的脉动流环境中剪切42 h.11 cm处理的内皮素-1(ET-1)平均分泌速率(16.93±0.89 pg/cm2*h)显著低于21 cm处理(26.13±1.79 pg/cm2*h), 差异比率为1∶1.5.脉动流作用引起的ET-1平均分泌率显著高于定常流的作用. 总体上表明在脉动流条件下,血管段长度与其血管内皮细胞ET-1代谢(分泌量)间有密切的相关关系.本实验结果从又一个侧面证实血管内皮细胞膜张应力存在累加效应.
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| 关 键 词： | 脉动流场 离体血管内皮细胞 张应力累加效应 内皮素 脉动层流 生物力学 |
Correlation of Length of Vascular Segment in vitro with Endothelin-1 Metabolism of Endothelial Cells-- Study on Accumulative Effect of Tensile Stress in the Upper Endothelial Cell Membrane Ⅲ |
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| Guixue Wang,Chenghao Xu,Shaoxi Cai,Qiang Feng,Yuanliang Wang. Correlation of Length of Vascular Segment in vitro with Endothelin-1 Metabolism of Endothelial Cells-- Study on Accumulative Effect of Tensile Stress in the Upper Endothelial Cell Membrane Ⅲ[J]. Journal of biomedical engineering, 2002, 19(4): 606-610 |
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| Authors: | Guixue Wang Chenghao Xu Shaoxi Cai Qiang Feng Yuanliang Wang |
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| Affiliation: | College of Bioengineering, Key Lab. for Biomechanics & Tissue Engineering Under the State Ministry of Education, Chongqing University, Chongqing 400044. |
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| Abstract: | A few of flow experiments were utilized to verify a theoretical hypothesis proposed by Fung and coworker which showed that the tensile stress in the upper cell membrane of the vascular endothelium could accumulate upstream against the direction of blood flow. Endothelial cells from replicate human umbilical vein segment (HUVSEC) in vitro with length of 11 cm and 21 cm were exposed to the same pulsatile laminar shear stress averaged of 0.12 N/m2 for 42 hours. The average production rate of endothelin-1(ET-1), at 11 cm segment is 50% lower than that at 21 cm segment(16.93 +/- 0.89) vs. (26.13 +/- 1.79) pg/cm2.h respectively. The average production rate of ET-1 under pulsatile laminar flow was significantly higher than that under steady laminar flow. It showed that, high correlation of the length of HUVSEC with their ET-1 metabolism exists, suggesting that the tensile stress in the upper endothelial cell membrane could accumulate. |
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