益肾胶囊对糖尿病肾病模型肾小球足细胞的影响

目的：观察益肾胶囊对糖尿病肾病（DN）大鼠肾组织病理改变及足细胞超微结构的影响。方法：将60只Wis-tar大鼠随机分为4组：正常对照组（对照组）、DN模型组（模型组）、苯那普利组、益肾胶囊组。于注射链脲佐菌素（STZ）后3d起,苯那普利组每日每只灌胃苯那普利3.125mg.kg-1.d-1,益肾胶囊组每日每只灌胃益肾胶囊625mg.kg-1.d-1,对照组及模型组每日给予等量的蒸镏水。各组分别干预12周,观察24h尿蛋白定量、血肌酐（Scr）、尿素氮（BUN）的变化,同时行肾脏病理检查。结果：12周末,模型组大鼠24h尿蛋白定量、Scr、BUN均高于对照组（P〈0.05）。苯那普利组及益肾胶囊组24h尿蛋白定量、Scr、BUN均低于模型组（P〈0.05）。光镜下模型组大鼠肾小球系膜基质增多,系膜区增宽;电镜下模型组大鼠肾小球基底膜增厚,足细胞排列紊乱,数目减少,足突增宽、融合。苯那普利组及益肾胶囊组肾小球基底膜病变减轻,细胞外基质减少,足细胞数目增多,足突融合减轻。结论：益肾胶囊能降低尿蛋白排泄,改善肾功能,并对足细胞有一定的保护作用,从而延缓大鼠糖尿病大鼠肾脏损害。

Effect of Yishe Capsule on Podocytes in Renal Tissue of Rats with Diabetic Nephropathy

Objective:To observe the effect of yishe capsule on pathological changes of renal tissue and podocyte ultrastruc-ture. Method:Totally 60 wistar rats were divided into four groups: normol control group (controf group),DN model group(model group) ,yishe capsule treatment gronp,benazepril treatment group.The rat in group of yishe capsule were garage given 625 mg.kg-1.water was used. AU the mrs received daily gavage for 12 weeks, the urinary protein in 24 h and the level of serum creatinine,urea ni-trogen were measured. The renal pathology and the changes of podocyte ultrastructure were detected under light microscope and transmission electron microscope. Results: In 12 w,urinary protein quantity in 24 h,Scr and BUN of model group rats were higher distinctly than those of control group( respectively, P < 0.05). After treatment of benazepril and yishe capsule, urinary protein quanti-ty in 24 h,Scr,BUN were reduced( respectively P < 0.05). We observed basement membrane thicken,foot process with disorders,foot process fusion,increased mesangial matrix, widened mesangium in model group by light microscope and transmission eletron mi-croscope. Benazepril and yishe capsule can improve ultrastructure in kidney of diabetic rats, lessen the fusion of foot process, ameliorate the renal pathomorphology change. Conclusion: Yishe capsule had a effect on the podocyte of diabetic nephropathy rats by reducing urine protein and improving renal function, thus alleviated the renal damage in diabetic rats.