| 腺病毒41型致缺乏腺病毒E_1区的A_(549)细胞产生病变的实验研究 |
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| 引用本文： | 张卫东,陈秀珠,张坚,沈英,张焰辉. 腺病毒41型致缺乏腺病毒E_1区的A_(549)细胞产生病变的实验研究[J]. 华中科技大学学报(医学版), 1997, 0(1) |
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| 作者姓名： | 张卫东 陈秀珠 张坚 沈英 张焰辉 |
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| 作者单位： | 同济医科大学基础医学院微生物学教研室!武汉，430030，同济医科大学基础医学院微生物学教研室!武汉，430030，同济医科大学基础医学院微生物学教研室!武汉，430030，同济医科大学基础医学院微生物学教研室!武汉，430030，同济医科大学基础医学院微生物学教研室!武汉，430030 |
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| 基金项目： | 国家自然科学基金!39370038 |
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| 摘 要： | 首次报道了腺病毒41型TaK株致缺乏腺病毒E1基因功能的A(549)细胞产生细胞病变。该病变与病毒感染的稀释度存在量一效关系。核酸杂交示1:10与1:20稀释度组病毒核酸含量相近，但明显高于1:50组。免疫荧光技术分析显示各稀释度组病毒蛋白表达起始时相均在病毒感染后的2～3d。
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| 关 键 词： | 肠道腺病毒41型 腺病毒.人 A_(549)细胞 腹泻.婴儿 |
A Study of Cytopathic Effect of Adenovirus Type 41 (TaK Strain) on A_(549) Cell Line Lacking E_1 Function of Adenovirus |
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| Zhang Weidong, Chen Xiuzhu, Zhang Jian et al. A Study of Cytopathic Effect of Adenovirus Type 41 (TaK Strain) on A_(549) Cell Line Lacking E_1 Function of Adenovirus[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1997, 0(1) |
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| Authors: | Zhang Weidong Chen Xiuzhu Zhang Jian et al |
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| Abstract: | The typical cytopathic effect (CPE) of adenovirus type 41 (TaK strain) on A549 cell line having no E1 function of adenovirus was described for the first time. The initial time and the extent of CPE on A549 cells were different among the various groups of virus dilptions. However, the beginning time of virus antigen expressed on A549 cells was likely to be the same 2 or 3 days after infection with the TaK strain. The result of molecular hybridization showed that the amount of nucleic acid of virus at groups of 1:10 or 1:20 dilution was greater than that group of 1: 50 dilution 12 days after being infected with TaK strain. |
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| Keywords: | enteric adenovirus type 41 adenovirus human A_(549) cell line diarrhoea infant |
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