幽门螺杆菌粪便抗原检测——一种新的非侵入性检查

目的：了解幽门螺杆菌粪便抗原捡测方法(Helicobacter pylori stool Antigen HpSA)的准确性与可靠性。方法：采用酶联免疫分析“双抗体夹心法”对303例因上消化道症状而进行胃镜检查的患的粪便标本进行幽门螺杆菌捡测,以快速尿素酶试验,组织学染色和培养捡测Hp,以三项检查中至少两项阳性或Hp培养捡测阳性判定为Hp感染作为“金标准。结果：“金标准”诊断Hp感染阳性182例,阴性121例。“金标准”阳性的182例中176例HpSA检测阳性,阴性的121例中100例HpSA检测阴性,HpSA敏感度为96．7％(176／182),特异度为82．6％(100／121)。结论：HpSA检测是一种简便易行,准确性高的非侵人性诊断Hp的方法,值得临床进一步推广。

The Helicobacter Pylori Stool Antigen Test--A New Non- invasive Test

Objective: To evaluate the reliability of an enzyme - linked immunosorbent assay by the Helicobacter pylori stool antigen test(HpSA) for the detection of H. pylori infection.Methods: Stool specimens were collected from 303 patients who received upper gastrointestinal endoseopic examination for gastrointestinal symptoms. H. pylori status was evaluated by four different tests: culture , histology, biopsy rapid urease test, HpSA test. Biopsy methods were considered gold standard . H. pylori status was defined as positive when two of the three biopsy tests were positive or the culture was positive. Results: 182 patients were diagnosed as H. pylori positive by "gold standard". HpSA test was positive in 197 patients an 1 negative in 106 patients. The sensitivity and specificity were 96.7% and 82.6% , respectively. The. overdll accuracy was 91.1%.Conctusion: The H. pylori stool test is a simple, non - invasive method for accurate diagnosis of H. pylori infection. It is worth spreading in clinical.