脑尔康对老年痴呆小鼠脑内胆碱酯酶活性及神经元的影响

目的 探讨复方中药脑尔康对小鼠学习记忆障碍的保护作用及其对胆碱酯酶活性的影响及保护神经元的抗痴呆作用机制.方法 健康昆明种雄性小鼠 60只,按随机数字表法分为 6组 ,除空白组外,连续造模50 d后,每组随机取 3只,用苏木精-伊红( HE)染色法,观察小鼠海马 CA1区细胞数量及形态,其余动物迅速断头取脑,冰台分离海马及皮质,检测各自的胆碱酯酶活性.结果 模型组胆碱酯酶活性明显增高 [脑皮质、海马模型组( 3.0± 0.1),(2.7± 0.1) mol/s;空白组( 2.2± 0.1) ,(2.2± 0.1)mol/s]( q=16.7,48.2,P< 0.01),用药各组与模型组比较,除小剂量组外胆碱酯酶活性均明显低于模型组(q=7.9～ 37.9,P< 0.01);模型组 [(47± 4)个 /200 μ m]海马 CA1区神经元数量明显少于空白组[(101± 11)个 /200 μ m]( q=5.39,P< 0.01),可见神经元脱失 ,并有胶质细胞增生.用药各组与模型组比较,仅有中药大、中剂量组神经元数量显著多于模型组(q=3.4,P< 0.05或 q=4.3,P< 0.01). 结论 脑尔康能明显降低老年痴呆模型小鼠皮层及海马胆碱酯酶活性,并有保护海马CA1区神经元的作用.

Influence of Naoerkang on the intracerebral cholinesterase activity and neurons of mice with Alzheimer''s disease

AIM:To study the effect of Naoerkang in protecting mice against learning and memory disorders and its influence on the cholinesterase activity and its mechanism of antidementia. METHODS:Sixty healthy male mice of Kunming species were randomized into 6 groups.Except the blank control group,after making model continuously for 50 d,3 mice were selected randomly from each group,with hematoxylin eosin staining,for observing the quantity and appearance of neurocytes in the hippocampal CA1 region.The heads of other rats were cut immediately with their brains taken out,separating the hippocampus and cortexes for testing the acetylcholinesterase activity. RESULTS:The acetylcholinesterase activity of model group was greatly strengthened[cerebral cortex and hippocampous:(3.0± 0.1) and(2.7± 0.1)mol/s in the model group;(2.2± 0.1)and (2.2± 0.1)mol/s in the blank group],(q=16.7,48.2,P< 0.01).To compare the medicinal groups with the model group,except the small dosage group,the acetylcholinesterase activity was much lower than that of the model group(q=7.9- 37.9,P< 0.01);the number of neurons in the hippocampal CA1 area of the model group[(47± 4)/200 μ m]was obviously less than that of the blank group[(101± 11)/200 μ m](q=5.39,P< 0.01),and the neuron loss and the gliocyte proliferation existed.Comparing the medicinal groups with the model group,only in the big and medium dosage groups,the number of neurons was obviously more than that of the model group(q=3.4,P< 0.05 or q=4.3,P< 0.01). CONCLUSION:Naoerkang is able to decrease the acetylcholinesterase activity in the cerebral cortex and hippocampus of AD model mice and protect the neurons in the hippocampal CA1 region.