| 饮用水中二溴乙酸遗传毒性研究 |
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| 引用本文： | 方城,王玉鹏,蒋颂辉,朱惠刚. 饮用水中二溴乙酸遗传毒性研究[J]. 卫生研究, 2001, 30(5): 266-269 |
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| 作者姓名： | 方城 王玉鹏 蒋颂辉 朱惠刚 |
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| 作者单位： | 复旦大学医学院, |
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| 基金项目： | 国家自然科学基金资助(No.39970628) |
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| 摘 要： | 二溴乙酸 (DBA)是饮用水氯化消毒副产物中的一种 ,属于氯乙酸类 ,臭氧消毒也可产生。本次试验采用Ames试验、程序外DNA合成 (UDS)试验、小鼠体内微核试验、NIH3T3细胞微核试验对其遗传毒性进行了研究。在Ames试验中 ,TA10 0菌株在加与不加S9的情况下均表现出致突变性。在大鼠肝细胞UDS试验中 ,DBA表现出明显的DNA损伤作用 ,导致程序外合成增加。在小鼠体内微核试验中 ,DBA在一定剂量范围内可引起染色体损伤。在NIH3T3细胞微核试验中 ,DBA可诱导细胞微核数增加。上述结果表示 ,DBA具有明确的DNA损伤作用。
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| 关 键 词： | 二溴乙酸 遗传毒性 Ames试验 程序外DNA合成试验 小鼠体内微核试验 NIH3T3细胞微核试验 |
| 文章编号： | 1000-8020(2001)05-0266-03 |
| 修稿时间： | 2000-10-30 |
Study on the genotoxicity of dibromoacetic acid in drinking water |
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| Fang Cheng,Wang Yu peng,Jiang Songhui,Zhu Huigang. Study on the genotoxicity of dibromoacetic acid in drinking water[J]. Journal of hygiene research, 2001, 30(5): 266-269 |
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| Authors: | Fang Cheng Wang Yu peng Jiang Songhui Zhu Huigang |
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| Affiliation: | Department of Environmental Health, Medical Center, Fudan University, Shanghai 200032, China. |
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| Abstract: | Dibroacetic acid (DBA) is a byproduct of drinking water disinfected by halogenation and it belongs to halogenated acetic acids. It also can be found in ozonated drinking water. Four short term assays were performed to detect the genotoxic activity of DBA. In the Ames test, DBA showed mutagenic activity in Salmonella typhimurium strain TA100 with or without rat liver derived metabolic activation system (S9). In UDS test, DBA was found to induce DNA damage in rat primary hepatocyte. Mouse bone marrow micronucleus test indicated that DBA induced the damage of chromosome.In NIH3T3cell micronucleus test, DBA increased the number of micronuclei in cells. Our results suggest that DBA induce DNA damage and has genotoxic activity. |
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| Keywords: | dibroacetic acid genotoxicity Ames test unscheduled DNA synthesis(UDS) test mouse bone marrow micronucleus test NIH3T3cell micronucleus test |
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