幽门螺杆菌热休克蛋白60诱导的抗原调节性T细胞及其对小鼠动脉粥样硬化的影响

目的 观察幽门螺杆菌热休克蛋白60(HP-HSP60)诱导的抗原特异性T细胞接种对ApoE~(-/-)小鼠动脉粥样硬化斑块的影响.方法 制备小鼠骨髓单核细胞,体外诱导HP-HSP60特异性调节性T细胞分化,并接种CD4~+CD25~+T_(reg)细胞后,观察其对小鼠动脉粥样斑块形成的影响.结果 阿司匹林处理的树突状细胞CDS0(43.0%)和CD86(41.1%)表达减少,形态学表现为未成熟树突状细胞,但其能诱导更多的特异性CD4~+CD25~+T_(reg)细胞(13.0±1.94)%,实验组粥样斑块面积(2.37±0.96)mm~2显著小于对照组(P<0.05).结论 未成熟树突状细胞可诱导出HSP60抗原特异性调节性CD4~+CD25~+T_(reg)细胞,后者在体内能明显抑制动脉粥样斑块的形成.

Effects of helicobacter pylori-HSP60-induced regulatory T cell on the progression of atherosclerotic plaque in mice

Objective To explore the induction of Helicobacter pylori-HSP60-induced CD4~+ CD25~+ T cell in vitro and its effect on the formation of atherosclerotic plaque. Methods Immature dendritic cells were extracted and induced from mouse bone marrow and then used to induce Helicobacter pylori-HSP60 CD4~+ CD25~+ T cells in vitro. After infusing the CD4~+ CIY25~+ T cells into homogenous mice, the size of atherosclerotic plaques was measured. Results Compared with control group, the expression of CDSO (43.0%) and CD86 (41.1%) on aspirin-treated dendritic cells was down-regulated. Immature dendritic cells induced more specific regulatory cells (13.0±1.94)% than the mature ones. These cells signiticantlty suppressed the proliferation. The size of atherosclerotic plaques (2.37±0.96) mm~2 in treated mice were smaller than untreated ones (P< 0.05). Conclusion Immature dendritic cells can be used to induce HSP60 specific regulatory T cells in vitro and the latter also can inhibit the progession of atherosclerosis.