一测多评法同时测定尖叶假龙胆中龙胆苦苷、芒果苷、当药醇苷、木犀草素、去甲基雏菊叶龙胆酮及雏菊叶龙胆酮

目的建立基于HPLC-UV技术的一测多评法(QAMS)同时测定尖叶假龙胆Gentianella acuta药材中雏菊叶龙胆酮、龙胆苦苷、芒果苷、当药醇苷、木犀草素和去甲基雏菊叶龙胆酮的含量。方法采用HPLC-UV技术,以雏菊叶龙胆酮为内参物,分别测定其与龙胆苦苷、芒果苷、当药醇苷、木犀草素和去甲基雏菊叶龙胆酮的相对校正因子,并计算含量;通过外标法与QAMS法分别对6种成分含量进行比较,以检验QAMS法的适用性、准确性和可重复性。结果 QAMS法与外标法所测得的结果无显著性差异。结论所建立的QAMS方法可满足尖叶假龙胆在缺乏对照品的情况下,完成尖叶假龙胆中多指标成分的同时测定,为尖叶假龙胆质量控制标准的建立提供依据。

Simultaneous determination of gentiopicroside, mangiferin, swertianolin, luteolin, demethylbellidifolin and bellidifolin in Gentianella acuta by QAMS method

Objective To detect the contents of six components in Gentianella acuta by establishing the method of quantitative analysis multi-components by single marker (QAMS). Methods Using HPLC-UV technology and taking bellidifolin as internal standard, the relative calibration factor of gentiopicroside, mangiferin, swertianolin, luteolin and demethylbellidifolin were detected respectively. The contents of the six measured components were compared by using the external standard method and the QAMS method, in order to verify the applicability, accuracy and repeatability of QAMS. Results There were no significant differences between QAMS method and external standard. Conclusion The established method can be used to simultaneous detect the multi-index components of G.acuta in the absence of relevant control, which could be helpful to establish the quality control specification of G.acuta.