uPA基因启动子甲基化与喉鳞状细胞癌的关系

目的：探讨尿激酶型纤溶酶原激活剂（uPA）基因启动子甲基化状况与喉癌侵袭转移的关系。方法：采用RT-PCR法检测40例喉癌组织中uPAmRNA表达；同时用甲基化特异性PCR（MSP）法检测uPA基因启动子甲基化状况。结果：uPA基因表达率为65．0％（26／40）；有颈淋巴结转移的喉癌与无颈淋巴结转移的喉癌比较，uPA基因表达率显著增高（P〈0．01）。uPA基因启动子甲基化率为20．0％（8／40）；有颈淋巴结转移的喉癌与无颈淋巴结转移的喉癌比较，uPA基因启动子甲基化率显著降低（P〈0．01）。甲基化的喉癌组织中均无uPA基因表达。结论：uPA基因启动子甲基化是uPA基因表达缺失的机制之一，uPA基因启动子的去甲基化机制可能与喉癌颈淋巴结转移有关。

Methylation status of uPA promoter in laryngeal squamous cell carcinoma

Objective:To investigate the relationship between methylation status of urokinase type plasminogen activator (uPA) promoter and invasion/metastasis of laryngeal squamous cell carcinoma (LSCC).Method:Using RT-PCR technique, specimens from 40 patients of LSCC were detected for their expression of the uPA gene. Meanwhile, methylation-specific PCR was used to detect methylation status of uPA promoter.Result:Positive expression rate of uPAmRNA in LSCC was 65.0%(26/40), and the rate of expression of uPAmRNA in LSCC with cervical lymph node metastasis was higher than that of in LSCC without cervical lymph node metastasis(P< 0.05 ).Methylation of uPA promoter was observed in 8 (20.0%) of 40 cases of LSCC, and the rate of methylation of uPA promoter in LSCC with cervical lymph node metastasis was lower than that of in LSCC without cervical lymph node metastasis(P<0.01).The expression of uPAmRNA was not detected in methylated cancer tissues.Conclusion:Methylation leads to lose of the uPA gene expression in LSCC. Demethylation of uPA promoter may be correlated to cervical lymph node metastasis.