| 致癌物诱导恶变细胞中线粒体高变区序列分析 |
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| 引用本文： | 傅娟,蒋义国,陈家堃,吴中亮,曹虹. 致癌物诱导恶变细胞中线粒体高变区序列分析[J]. 中国公共卫生, 2006, 22(7): 816-817 |
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| 作者姓名： | 傅娟 蒋义国 陈家堃 吴中亮 曹虹 |
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| 作者单位： | 1. 广州医学院预防医学系与化学致癌研究所,广州,510182
2. 江西省人民医院 |
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| 基金项目： | 国家自然科学基金;广东省博士启动基金;广东省医学科研项目 |
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| 摘 要： | 目的检测苯并（a）芘代谢产物反式二羟环氧苯并芘（反式-BPDE）及结晶型硫化镍（NiS）诱发永生化人支气管上皮细胞系（16HBE）恶性转化过程中线粒体DNA控制区序列变化。探讨线粒体DNA控制区在环境致癌物苯并（a）芘及硫化镍致癌作用中是否存在靶分子序列。方法 应用银染PCR-单链构向多态性（SSCP）方法及测序方法分析恶变细胞中线粒体DNA高变区的序列变化。结果 经反式-BPDE和NiS诱导恶变的人支气管上皮细胞系线粒体高变区均未发现异常改变。结论 线粒体基因控制区可能并非是化学致癌物诱发肺癌的靶序列。
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| 关 键 词： | 苯并(a)芘 硫化镍 人支气管上皮细胞 线粒体基因 |
| 文章编号： | 1001-0580(2006)07-0816-02 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide |
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| FU Juan, JIANG Yiguo, CHEN Jiakun,et al.. Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide[J]. Chinese Journal of Public Health, 2006, 22(7): 816-817 |
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| Authors: | FU Juan JIANG Yiguo CHEN Jiakun et al. |
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| Affiliation: | FU Juan, JIANG Yiguo, CHEN Jiakun, et al . |
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| Abstract: | Objective To explore the alteration of target molecular sequences in the noncoding mitochondrial DNA(mtDNA) control region in 16HBE cells transformed by carcinogens trans-BPDE and crystalline nickel sulfide.Methods The sequences of mt DNA control region in 16HBE cells were detected by PCR-SSCP assay and sequencing.Results Compared with those of control 16HBE cells,no change in hypervariable segments of mtDNA in malignant transformed cells and tumorigenic cells induced by trans-BPDE and crystalline nickel sulfide was detected.Conclusion The noncoding mtDNA control region might not be a target sequence in carcinogenesis induced by chemical carcinogen. |
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| Keywords: | benzo(a)pyrene nickel sulfide human bronchial epitheial cell line mitochondrial gene |
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