眼睑原发性恶性肿瘤组织P21~(waf1/cip1)蛋白的表达及其意义

目的:分析抑癌基因表达产物P21waf1/cip1蛋白在眼睑原发性恶性肿瘤（Eye-lip　Primary　MalignantTumor,E-LPMT）组织中的阳性表达情况。方法:采用人P21waf1/cip1蛋白单克隆抗体,以免疫组化streptavidinbiotin　peroxidase　complex（SP）染色方法检测眼睑原发性恶性肿瘤组织病理切片。结果:P21waf1/cip1蛋白阳性表达于部分眼睑原发性恶性肿瘤组织细胞核中,61例患者病理切片染色后得出不同类型的眼睑原发性恶性肿瘤组织P21waf1/cip1蛋白阳性表达率:基底细胞癌组织P21waf1/cip1蛋白阳性表达率大于鳞状上皮癌组织P21waf1/cip1蛋白阳性表达率（P<0.01）;鳞状上皮癌组织P21waf1/cip1蛋白阳性表达率大于睑板腺癌组织P21waf1/cip1蛋白阳性表达率（P<0.05）,且相比均有显著性差异。分化程度高的乳头状癌和囊腺癌组织P21waf1/cip1蛋白阳性表达率大于分化程度低的结节型肿瘤组织组织P21waf1/cip1蛋白阳性表达率（P<0.01）。结论:抑癌基因表达产物P21waf1/cip1蛋白阳性表达率与眼睑原发性恶性肿瘤组织的分化程度相关。对临床治疗及预后具有指导意义。眼科学报2001;17:206～208。

Expression Significance of P21~(waf1/Ccip1) Protein in Eye-lip Primary Malignant Tumor

Perpose: To study the expression of p21wafl/cipl protein in eye-lip primary malignant tumor (E-LPMT).Methods: Immunohistochemical streptavidin biotin peroxidase complex (SP) method was used to stain section of E-LPMT.Results: In 61 cases of E-LPMT, positive expression of P21wafl/cipl protein was found locating in the nuclei. In Cell carcinoma, Squamous and Adenocarcinomas, the positive rate of P21wafl/cipl protein expression were 36. 0% , 27. 8% and 22. 2% respectively. The positive rate of P21wafl/cifl protein expression in well-differentiated Cell carcinomas or Adenocarcinomas was higher than in poor-differentiated ones (P<0.01). Conclusion: P21wafl/cipl protein expresses in various type of E-IPMT and its positive expression in Cell carainomas and Adenocarcinomas are related to their different degrees. Eye Science 2001; 17: 206 ~ 208.