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Effects of Efflux Pump Inhibitors on Colistin Resistance in Multidrug-Resistant Gram-Negative Bacteria |
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| Authors: | Wentao Ni Yanjun Li Jie Guan Jin Zhao Junchang Cui Rui Wang Youning Liu |
| Affiliation: | aDepartment of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China;bDepartment of Clinical Laboratory, Navy General Hospital, Beijing, China;cDepartment of Clinical Laboratory, Peking University First Hospital, Beijing, China;dDepartment of Clinical Pharmacology, Chinese PLA General Hospital, Beijing, China |
| Abstract: | We tested the effects of various putative efflux pump inhibitors on colistin resistance in multidrug-resistant Gram-negative bacteria. Addition of 10 mg/liter cyanide 3-chlorophenylhydrazone (CCCP) to the test medium could significantly decrease the MICs of colistin-resistant strains. Time-kill assays showed CCCP could reverse colistin resistance and inhibit the regrowth of the resistant subpopulation, especially in Acinetobacter baumannii and Stenotrophomonas maltophilia. These results suggest colistin resistance in Gram-negative bacteria can be suppressed and reversed by CCCP. |
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