肝纤维化时基质金属蛋白酶-2及其组织抑制因子的表达

目的：了解基质金属蛋白酶-2（MMP-2）及其组织抑制因子（TIMP-2）在不同病因致有肝纤维化病理阶段中的变化。分析MMP-2/TIMP-2与肝病理损害的关系。方法：用免疫组织化学（LsAB法），在正常肝组织，慢活肝伴早期肝纤维化，肝癌伴早期肝纤维化、胆汁性肝硬化、坏死后性肝硬化病例中MMP-2及TIMP-2的联合检测，行半定量研究。结果：正常肝组织MMP-2/TIMP-2的比值高于病肝组；早期肝纤维化组MMP-2/TIMP-2比值高于肝硬化组；早期肝纤维化组组间，肝硬化组组间MMP-2/TIMP-2比值无显著差异。结论：早期肝纤维化MMP-2表达相对增高，对肝纤维化的始动和进展起重要作用。肝硬化时TIMP-2表达明显增高，抑制MMP-2，细胞外基质（ECM）降解障碍而大量沉积，加重肝病理损害。

Expression of Matrix Metalloproteinase-2 and It's Tissue Inhibitor in Human Liver Fibrosis

Objective:To observe the changes of metalloproteinase-2(MMP-2)and its tissue inhibitor in human liver fibrosis from different causetive factors and at different stages,and to clarify relationship between MMP-2/TIMP-2 rate and liver pathology.Methods:Using immunohistochemistry,the expression of MMP-2 and TIMP-2 were detected in human liver fibrosis during every stage of liver pathology.Results:1 The MMP-2/TIMP-2 rate shoes no higher in corpse liver than in liver diseases.2 The MMP-2/TIMP-2 rate was higher in the early stage of liver fibrogenesis than in cirrhosis.3 The MMP-2/TIMP-2 rate was not different between the active chronic hepatitis with the early stage of liver fibrogenesis and hepatocellular carcinoma with the early stage of liver fibrogenesis as well as between biliary cirrhosis and postnecrotic cirrhosis.Conclusions:It is important that the expression of MMP-2 was relatively increased in the early stage of liver fibrogenesis and its progression.The expression of TIMP-2 was extraordinary increased in cirrhosis to inhibit MMP-2,and to result in the accumulation of ECM in liver.