Leflunomide抑制大鼠角膜移植免疫排斥反应的研究

目的研究Leflunomide对大鼠角膜移植排斥反应的防治作用。方法建立大鼠穿透性角膜移植排斥反应的动物模型，观察Leflunomide对大鼠角膜植片存活和排斥反应指数（RI）的影响，并与阴性对照组和CsA治疗组相比较。结果阴性对照组角膜植片存活时间为12.375d±1.768d,而CsA组为17.375d±1.408d,Leflunomide组为18.250d±1.356d，均比阴性对照组显著延长（P<0.01）。结论Leflunomide能抑制大鼠穿透性角膜移植免疫排斥反应，显著延长角膜植片的存活时间。

Effects ofimmunosuppressant Leflunomide on penetrating keratoplasty rejection model in rat

Objective　 To study the efficacy of immunosuppressant Leflunomide on the rat model of penetrating keratoplasty.Methods　 Corneal grafts were transplanted orthotopically from Lou rats to F344 rats recipients. Animals were randomly assigned to the following groups: (1) control; (2) CsA (5mg*kg-1*d-1, po); (3) Leflunomide (5mg*kg-1*d-1, po). Treatment was begun on the first postoperative day and continued until the 14th day . Mean survival times and rejection index were determined.Results　The mean survival time of the control group was merely 12.375 d±1.768d, But the mean survival time of the treated groups with CsA and Leflunomide were respectively 17.375 d ±1.408 d and 18.250 d±1.356 d,which was statistically prolonged compared with the control group(P<0.01).Conclusion　Leflunomide can markedly prolongs the survival time of corneal allografts by inhibiting graft rejection.