CCR7活化与人结肠癌SW620细胞的体外增殖、趋化与侵袭的关系

目的研究CCR7活化对结肠癌SW620细胞体外增殖、趋化与侵袭活性的影响。方法MTT法和软琼脂细胞集落培养观察CCR7活化对细胞增殖的影响，Boyden小室法检测CCR7活化对SW620细胞趋化和侵袭活性的影响。结果和对照组相比CCL21组细胞增殖数量、软琼脂细胞集落数和穿过Boyden小室膜的细胞数均显著增加（P〈0．01）。结论CCR7活化能够促进结肠癌SW620细胞的体外增殖、趋化与侵袭，其可能参与了结肠癌淋巴结转移的过程。进一步研究CCR7在结肠癌中的作用将有助于阐明结肠癌淋巴结转移的机制。

The relationship between CCR7 activation and proliferation,chemotaxis and invasion of human colon cancer SW620 cells in vitro

Objective To investigate the effects of CCR7 activation in vitro proliferation,chemotaxis and invasion of human colon cancer SW620 cells. Methods SW620 proliferation ability was examined by MTT assay and plate clone formation assay. Chemotaxis and invasion of SW620 cells were investigated using Boyden chamber assay with the absence and presence of CCL21. Results Compared with those in the control group,the numbers of the proliferative cells ,forming plate cells clones and cells permeating the septum of the Boyden chamber were significantly increased in the CCL21 group （P 〈 0. 01 ）. Conclusions CCR7 activation could promote proliferation, chemotaxis and invasion of SW620 cells, and it maybe play a role in lymph node metastasis of colon cancer. To further study CCR7 may help to elucidate the mechanism of lymph node metastasis in human colon cancer.