多巴胺、硝酸甘油诱导血管舒缩异常SD大鼠模型的建立

目的:筛选合适的多巴胺(DA)提前给药时间,建立先后应用DA和硝酸甘油(GTN)诱导的血管舒缩异常大鼠模型。方法:大鼠分为5组:正常组、DA组、根据DA不同时间点(10,30,45min)加GTN等3组,通过脉冲多普勒血流计对大鼠颈总动脉血流速度动态检测,评价DA、GTN对血流速度变化率的影响。结果:给药组的DA在注射后的10min内血流速度变化率与正常组同时间点组间比较有差异(P<0.05),但是从11min起与正常组组间比较差异不显著(P>0.05)。10min间隔组血流速度变化率与DA组在45,50,55,60,65,70min等6个时间点比较差异有统计学意义(P<0.05或P<0.01)。45min间隔组颈总动脉血流速度变化率与DA组在80,85,90,95,100min等5个时间点比较差异有统计学意义(P<0.05)。30min间隔组颈总动脉血流速度变化率在各个时间点与DA组比较差异没有统计学意义(P>0.05)。结论:皮下注射DA、间隔45min再注射GTN可建立具有时相性特点的血管舒缩异常大鼠模型。该模型有可能用于防治偏头痛药物的血管活性筛选。

Establishment of a SD Rat Model with Vasomotor Abnormalities Induced by Dopamine and Nitroglycerin

Objective:To determine the appropriate time of early administration of dopamine(DA) before nitroglycerin(GTN) for establishing rat model with vasomotor abnormalities.Methods: Rats were divided into 5 groups: normal group,DA Group and other 3 groups in accordance with DA at different time points(10,30,45 min) plus GTN.Common carotid artery(CCA) blood flow velocity was dynamically measured with pulsed Doppler flow probe to evaluate the influence of DA,GTN on blood flow velocity rate(BFVR).Results:The blood flow velocity rate induced by DA of All medication administration was different from the normal group within 10 minutes(P<0.05).However,the differences were not statistically significant after 11 min(P>0.05).The BFVR of 10min interval group had differences with the DA group at 45min,50min,55min,60min,65min and 70min(P<0.05 or P<0.01).CCA BFVR of 45 min interval group was statistically significant different from the DA group at 80,85,90,95 min and 100 min(P<0.05).No statistically significant differences existed between CCA BFVR of 30min interval group and DA group(P>0.05).Conclusion: Subcutaneous injection of DA,45min after injection of GTN can induce vasomotor abnormalities in rat with phase characters.The model may be useful for vasoactive screening of anti-migraine drugs.