大肠癌组织中PTEN和VEGF蛋白的表达及相关性研究

目的探讨大肠癌组织中肿瘤抑制基因(PTN)和血管内皮生长因子(VEGF)蛋白表达的意义.方法采用免疫组化SP法检测80例大肠癌中PTEN和VEGF蛋白的表达,并分析其与临床和病理特点的关系.结果大肠癌中PTEN蛋白表达阳性率为46.3%(37/80),显著低于正常大肠黏膜组织的阳性率(P＜0.01);VEGF蛋白表达阳性率为77.5%(62/80),显著高于正常大肠黏膜组织的阳性率(P＜0.01);PTEN蛋白低表达和VEGF蛋白高表达与大肠癌Dukes分期、浆膜浸润、淋巴结转移有关(均P＜0.01),而与年龄和组织学类型无关(均P＞0.05);PTEN和VEGF蛋白表达呈负相关(P＜0.01).结论PTEN和VEGF蛋白表达与大肠癌临床病理特征和生物学行为有密切关系,联合检测PTEN和VEGF蛋白的表达,可用于评估大肠癌侵袭转移能力,对大肠癌的预后判断具有一定的临床意义.

Study of correlation between protein expression of PTEN and VEGF in colorectal carcinoma

Objective To investigate the significance of tumor suppressor gene PTEN and vascular endothelial growth factor(VEGF) protein expression in colorectal adenocarcinoma. Methods The expression of PTEN and VEGF protein was examined with immunohistochemical SP method. Results The total positive rates of PTEN and VEGF protein staining in 80 cases of colorectal adenocarcinoma were46.3%(37/80)and 77.5%(62/80)respectively. They were not significantly correlated with Patient's age or histological type(P>0.05). The low level of PTEN protein expression and the high level of VEGF protein expression were correlated with the Dukes stage, serosa infiltration, lymph node metastasis of colorectal adenocarcinoma(P<0.01). The expression of PTEN protein was negatively correlated with that of VEGF protein(P<0.01). Conclusion The expressions of PTEN and VEGF protein were significantly correlated with the clinicopathological characteristics and biologic behaviors in colorectal adenocarcinoma. The combined detection of PTEN and VEGF protein expression may be helpful to evaluate prognosis and infiltrative capability of colorectal adenocarcinoma.