牛磺酸对幼鼠脑神经保护作用和机制研究

目的　观察牛磺酸 (Tau)对幼鼠脑神经的保护作用 ,并对其可能机制进行探讨。方法　用 0 .1 μmol/L亚硒酸钠与不同浓度的 Tau共同加入到原代培养 2 d的新生小鼠大脑皮质神经元中 ,用 MTT检测和 DNA片段琼脂糖凝胶电泳法分析其保护性作用 ;并选用健康清洁级、断乳SD雌鼠 3 2只 ,按体重随机分为 4组 ,分别饲予补充不同水平 Tau的实验饲料 ,观察对照组及补充Tau组幼鼠脑发育及代谢的影响。结果　 1 .Tau可明显提高神经元的存活率 ;2 .一定浓度的 Tau可阻断或部分阻断由亚硒酸钠诱导的典型 DNA梯型 ;3 .Tau可促进脑细胞的增殖活性 ,增加脑重 ;4.补充 Tau能明显提高脑 Tau积累、蛋白质和 ACh E水平 ,且有一定的剂量反应关系。结论　补充 Tau具有较强的神经保护作用 ,可能是通过改变某些蛋白质即某些基因的表达而实现的。

NEUROPROTECTION EFFECT OF TAURINE IN YOUNG RATS AND THE MECHANISM INVOLVED

Objective: To investigate the possible mechanism of neuroprotection produced by taurine supplement on brain in young rats. Methods: In vitro, taurine treatment or co-treatment with sodium selenite that produced significant neuronal apoptosis was used in cultured cortical neurons to examine the neuroprotection effect using MTT assay and DNA fragmentation electrophoresis. In vivo, female weanling Sprague Dawley rats were divided into four groups, eight rats per group. The treated groups were fed on diets with different contents of taurine respectively, and the control group was fed on taurine unsupplemented diets. The experiment lasted five weeks. Results: Taurine significantly increased neuronal survival in dose-dependent manner. The typical DNA ladder pattern could be prevented by taurine. Taurine could elevate brain wet weight, and significantly increased the level of taurine, protein and AChE in brain. Conclusion: It is proposed that the neuroprotection effect of taurine could be achieved by modulating expression of related genes and blocking neuronal apoptosis.