Partial deletion of p75NTR in large-diameter DRG neurons exerts no influence upon the survival of peripheral sensory neurons in vivo |
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Authors: | Zuoheng Qin David G Gonsalvez Rhiannon J Wood Fatemeh Daemi Sangwon Yoo Jason J Ivanusic Elizabeth J Coulson Simon S Murray Junhua Xiao |
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Institution: | 1. Department of Anatomy and Neuroscience, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, Australia;2. School of Biomedical Sciences, Queensland University, Brisbane, QLD, Australia |
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Abstract: | The p75 neurotrophin receptor (p75NTR) is required for maintaining peripheral sensory neuron survival and function; however, the underlying cellular mechanism remains unclear. The general view is that expression of p75NTR by the neuron itself is required for maintaining sensory neuron survival and myelination in the peripheral nervous system (PNS). Adopting a neuronal-specific conditional knockout strategy, we demonstrate the partial depletion of p75NTR in neurons exerts little influence upon maintaining sensory neuron survival and peripheral nerve myelination in health and after demyelinating neuropathy. Our data show that the density and total number of dorsal root ganglion (DRG) neurons in 2-month-old mice is not affected following the deletion of p75NTR in large-diameter myelinating neurons, as assessed by stereology. Adopting experimental autoimmune neuritis induced in adult male mice, an animal model of demyelinating peripheral neuropathy, we identify that deleting p75NTR in myelinating neurons exerts no influence upon the disease progression, the total number of DRG neurons, and the extent of myelin damage in the sciatic nerve, indicating that the expression of neuronal p75NTR is not essential for maintaining peripheral neuron survival and myelination after a demyelinating insult in vivo. Together, results of this study suggest that the survival and myelination of peripheral sensory neurons is independent of p75NTR expressed by a subtype of neurons in vivo. Thus, our findings provide new insights into the mechanism underpinning p75NTR-mediated neuronal survival in the PNS. |
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Keywords: | BDNF DRG EAN myelination p75NTR peripheral nervous system remyelination RRID:AB_11205760 RRID:AB_2801575 RRID:AB_2267254 RRID:AB_304560 RRID:AB_1658411 RRID:AB_2340380 RRID:AB_2732073 RRID:AB_2534102 RRID:AB_2340375 RRID:AB_2687445 RRID:AB_11205760 |
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