Mac ELISA测定乙脑患者血清和脑脊液中特异性IgM的评价

用抗体捕捉ELISA法(Mac ELISA)检测临床疑似流行性乙型脑炎(下称乙脑)病人脑脊液88份,阳性51份,阳性率57.95％;血清91份,阳性56份,阳性率为61.53％,两者无显著性差异(X~2=0.238 P>0.05);包括取自同一患者的脑脊液和血清各72份,阳性率分别为61.11％和68.05％;而分析其中14例IgG呈4倍以上增高,乙脑患者脑脊液IgM阳性13例,而血清IgM阳性却只有8例,提示在疫区流行季节内仅仅血清学IgM阳性有时可能为乙脑隐性感染的同时还伴其它有关病毒感染。

Evaluation of Mac ELISA Assay for Detection IgM Antibody of Japanese Encephalitis in Cerebrospinal Fluid and Sera

This paper reports An IgM capture ELISA for detection Japanese encephalitis IgM antibody in cerebrospinal fluid (CSF) and sera.The positive rate in CSF was 57.95% (51/88),and in sera 61.53% (56/91); 72 patients whose CSF and sera were takenout within 24h were also tested,with the positive rate in CSF and sera 61.11% and 68.05% respectively; 14 cases were with fourfold increasing in titer of IgG,in which positive in CSF was 13,and in sera 8.The results suggested that the positivity of IgM in sera during Japanese encephalitis epidemic season should be caused by subclinical infection and other relevant virus infection.