| 血清剥夺致PC12细胞死亡及对细胞周期的影响 |
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| 引用本文： | 潘邓记,张旻,杨明山. 血清剥夺致PC12细胞死亡及对细胞周期的影响[J]. 华中科技大学学报(医学版), 2004, 33(5): 566-568 |
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| 作者姓名： | 潘邓记 张旻 杨明山 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院神经内科,武汉,430030 |
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| 摘 要： | 目的 观察血清剥夺致PC12细胞死亡及对细胞周期的影响。方法 流式细胞术检测在含5％和2％胎牛血清的培养基中，PC12细胞死亡和细胞周期的改变。结果 随着血清浓度的下降。PC12细胞死亡或凋亡增加，同时，S期的细胞所占百分比降低，G2期细胞所占的比例增加，在含2％胎牛血清的培养基中培养的细胞更加明显。结论 ①PC12细胞经血清剥夺培养后产生的损伤可能引发了细胞周期阻滞，当损伤未得以及时修复时，导致细胞凋亡。②在细胞周期和细胞死亡之间可能存在密切的联系。
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| 关 键 词： | PC12细胞 血清剥夺 细胞周期阻滞 细胞死亡 胎牛血清 损伤 培养基 细胞凋亡 |
| 修稿时间： | 2003-05-31 |
Changes of Cell Cycle and Cell Death in PC12 Cells in Serum-deprivation Medium |
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| Pan Dengji,Zhang Min,Yang Mingshan. Changes of Cell Cycle and Cell Death in PC12 Cells in Serum-deprivation Medium[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2004, 33(5): 566-568 |
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| Authors: | Pan Dengji Zhang Min Yang Mingshan |
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| Affiliation: | Pan Dengji,Zhang Min,Yang Mingshan Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective To observe the changes of cell death and cell cycle of PC12 cells in serum-deprivation medium. Methods Flow cytometry was used to detect the changes of cell death and cell cycle of PC12 cells cultured in medium containing 5 % and 2 % serum. Results As the concentrations of serum were decreased, the percentage of dead or apoptotic cells was increased. At the same time, there was a decline in the percentage of cells in S phase and an increase in the percentage of cells in G_2. Such changes of cell cycle were more obvious in the cells cultured in medium containing 2 % serum. Conclusion The damage of PC12 cells induced by the serum deprivation led to cell cycle arrest, even the apoptosis when the damage couldn't be repaired on time. There may be a close relationship between cell cycle and cell death. |
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| Keywords: | pheochromocytoma PC12 cell serum deprivation cell death cell cycle |
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