| 早孕妇女活动性巨细胞病毒感染的调查及其宫内感染检测方法的研究 |
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| 引用本文： | 崔应琦,董兆文,伦玉兰,韩维田,王毅. 早孕妇女活动性巨细胞病毒感染的调查及其宫内感染检测方法的研究[J]. 中国计划生育学杂志, 1994, 0(6) |
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| 作者姓名： | 崔应琦 董兆文 伦玉兰 韩维田 王毅 |
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| 作者单位： | 国家计划生育委员会科学技术研究所，国家计划生育委员会科学技术研究所，青岛市计划生育研究所，辽宁省计划生育研究所，国家计划生育委员会科学技术研究所 100081，100081，100081 |
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| 摘 要： | 本文对北京、青岛及沈阳三市的1202名早孕妇女进行了原发性HCMV感染的临床调查,血清中特异性HCMV—IgM和IgA抗体的酶联免疫吸附法检测;分析了患病的风险因素;并采用辣根过氧化物酶复合物(PAP)免疫组化技术检测其胚胎绒毛中的HCMV,以便建立一种宫内感染诊断的方法。利用透射电镜和聚合酶链反应(PCR)技术来验证此方法的可靠性,结果显示PAP法的阳性及阴性率与电镜观察的符合率为100％,与PCR法的符合率分别为97.0％与86.6％,实为一种敏感、经济、易行的宫内诊断方法。
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| 关 键 词： | 人巨细胞病毒 人绒毛 宫内感染 特异性免疫球蛋白M类 特异性免疫球 蛋白A类 辣根过氧化物酶复合物 |
Investigastion of Active Human Cytomegalovirus Infection in Early Pregnant Wonen and the of Diagnostic Method of its Intrauterine Infection. |
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| Cui Ying-qi,National Research Institute for Family Planning Beijing,.. Investigastion of Active Human Cytomegalovirus Infection in Early Pregnant Wonen and the of Diagnostic Method of its Intrauterine Infection.[J]. Chinese Journal of Family Planning, 1994, 0(6) |
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| Authors: | Cui Ying-qi National Research Institute for Family Planning Beijing . |
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| Affiliation: | Cui Ying-qi,National Research Institute for Family Planning Beijing,100081. |
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| Abstract: | In order to study the prenatal diagnotic method of intrauterine human cytomegalovirus (HCMV) in-fection and to analyze its risk factors, 1202 early pregant women and their embryos in Beijing,Qing-dao and Shenyang were studied using ELISA and Peroxidase-Antiperoxidase (PAP ) method to test the HCMV specific antibody (IgM,IgA)in pregnant women and the HCMV antigen in their embryos. The reliability of PAP method was confirmed by electron microscope and polymerase Chain Reaction (PCR)technique. The result showed that the coinci-dence of positive and negative rates of PAP were both 100% with electron microscope findings (97. 0%and 86. 6% with PCR respectively. PAP method is a sensitive,cheap and easier method for intrauter-ine diagnosis. |
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| Keywords: | Human cytomegalovirus Peroxidase- Antiperoxidase ( PAP ) intrauterine Infection |
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