| 扫描电镜观察体内留置双J管表面生物膜的形成 |
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| 引用本文： | 米华,朱莲娜,梁宏洁,颜海标.扫描电镜观察体内留置双J管表面生物膜的形成[J].中华医院感染学杂志,2010,20(2). |
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| 作者姓名： | 米华 朱莲娜 梁宏洁 颜海标 |
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| 作者单位： | 1. 广西医科大学第一附属医院泌尿外科,广西南宁,530021
2. 广西医科大学第一附属医院检验科,广西南宁,530021 |
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| 摘 要： | 目的观察植入人体内的双J管表面生物膜的形成情况。方法对42例泌尿外科手术后植入人体内的双J管分成4段分别作细菌学检验,然后用扫描电镜观察其表面生物膜的形成。结果42份双J管标本中,4段双J管均有同种细菌生长20例(47.6%),各段双J管细菌培养阳性率分别为:肾盂段81.0%、输尿管上段64.3%、输尿管下段71.4%和膀胱内段83.3%,共培养出18种126株细菌,以大肠埃希菌(23.0%)、铜绿假单胞菌(15.9%)、棒杆菌属(15.1%)和表皮葡萄球菌(13.5%)多见;电镜扫描双J管表面观察到未经使用过的双J管表面呈凹凸不平,但干净无任何附着物或结晶体,植入体内的双J管表面均有数量不等的炎性附着物或结晶体,可观察到细菌结构,生物膜的细菌被包裹在大量的纤维膜之中。结论植入体内的双J管表面易形成细菌生物膜,这可能是导管相关感染的因素之一。
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| 关 键 词： | 细菌生物膜 双J管 扫描电镜 |
Bacterial Biofilm Formation on Indwelling Double J Stent Observed by Scanning Electron Microscopy |
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| MI Hua,ZHU Lian-na,LIANG Hong-jie,YAN Hai-biao.Bacterial Biofilm Formation on Indwelling Double J Stent Observed by Scanning Electron Microscopy[J].Chinese Journal of Nosocomiology,2010,20(2). |
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| Authors: | MI Hua ZHU Lian-na LIANG Hong-jie YAN Hai-biao |
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| Institution: | MI Hua,ZHU Lian-na,LIANG Hong-jie,YAN Hai-biao(The First Affiliated Hospital,Guangxi Medical University,Nanning,Guangxi 530021,China) |
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| Abstract: | OBJECTIVE To observe the bacterial biofilm formation on indwelling double J stent in the human body.METHODS A prospective study of 42 cases with double J stent indwelling in the human body after operation was conducted.The stent was divided into 4 segments then detected microbiologicaly in a separate manner and observed with scanning electron microscope for the presence of bacterial biofilm.RESULTS From 42 cases with douple J stent,only one case was without any bacteria in four segments.20 cases(47.6%) had ... |
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| Keywords: | Bacterial biofilm Double J stent Scaning electron microcopy |
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