A Simple Technique for Preparation of Bone Marrow or Peripheral Blood Buffy Coat Cells for Electron Microscopy |
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| Authors: | A. E. Mills Malcolm Emms S. G. Licata |
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| Affiliation: | a Department of Pathology Red Cross War Memorial Children's Hospital, Cape Town and the University of Cape Town, South Africa |
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| Abstract: | A simple and rapid method of processing ethylene diamine-tetra-acetic acid anticoagulated peripheral blood or aspirated bone marrow for electron microscopy is described. The resultant buffy coat pellet is easily processed into epoxy resin. Semithin sections (1 μ) stained with 1% toluidine blue reveal the various stratified cell layers allowing convenient selection for ultramicrotomy and ultrastructural evaluation. |
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| Keywords: | electron microscopy bone marrow aspirate peripheral blood buffy coat pellet |
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