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| 细丝蛋白A抑制荷人结肠腺癌细胞SW480裸鼠移植瘤的生长 | |
| 引用本文： | 史建伟,于跃明,王士杰,王小玲,王贵英.细丝蛋白A抑制荷人结肠腺癌细胞SW480裸鼠移植瘤的生长[J].肿瘤,2011,31(8):701-706. |
| 作者姓名： | 史建伟 于跃明 王士杰 王小玲 王贵英 |
| 作者单位： | 1. 河北大学附属医院肿瘤外科,保定,071000;河北医科大学第四医院外科,石家庄,050011 2. 河北医科大学第四医院外科,石家庄,050011 3. 河北医科大学第四医院内镜室,石家庄,050011 4. 河北医科大学第四医院病理科,石家庄,050011 |
| 基金项目： | 河北省卫生厅医学科学研究重点课题(编号:20090010)河北省教育厅高校强势特色学科建设基金 |
| 摘 要： | 目的:探讨细丝蛋白A(fi lamin A,FLNa)基因对人结肠腺癌SW480细胞在裸鼠体内成瘤的影响及其可能的机制。方法:将重组载体质粒pcDNA3.1/V5-His-TOPO/FLNa通过脂质体介导的方式转染到SW480细胞中,RT-PCR和蛋白质印迹法检测SW480/FLNa细胞中FLNa mRNA和蛋白的表达,MTT法检测细胞的体外增殖能力。将SW480/FLNa、SW480/pcDNA3.1和SW480细胞接种于裸鼠皮下,观察移植瘤的生长情况,计算抑瘤率,HE染色观察移植瘤组织的形态学变化,RT-PCR和蛋白质印迹法检测移植瘤组织中基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)mRNA和蛋白的表达水平。结果:FLNa基因在SW480细胞中获得稳定表达,SW480/FLNa、SW480/pcDNA3.1和SW480细胞的体外增殖能力相似;SW480/FLNa组移植瘤的生长速度和质量低于SW480组,抑瘤率为(88.7±3.5)%(P=0.000);SW480/FLNa组移植瘤组织出现退变坏死,MMP-9mRNA和蛋白的表达水平(0.11±0.02和0.02±0.00)均低于SW480组(1.12±0.02和1.25±0.05),差异均有统计学意义(P=0.012,P=0.025)。结论:FLNa基因具有抑制人结肠腺癌SW480细胞在裸鼠体内的生长作用,其作用机制可能与下调MMP-9的表达有关。 |
| 关 键 词： | 结直肠肿瘤 小鼠 裸 基因 肿瘤抑制 细丝蛋白A 细胞 SW480 |
Inhibitory effect of hlamin A on tumor formation activity of human colon carcinoma SW480 cells in nude mice |
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| SHI Jian-wei,YU Yue-ming,WANG Shi-jie,WANG Xiao-ling,WANG Gui-ying.Inhibitory effect of hlamin A on tumor formation activity of human colon carcinoma SW480 cells in nude mice[J].Tumor,2011,31(8):701-706. | |
| Authors: | SHI Jian-wei YU Yue-ming WANG Shi-jie WANG Xiao-ling WANG Gui-ying |
| Institution: | SHI Jian-wei1,2,YU Yue-ming2,WANG Shi-jie3,WANG Xiao-ling4,WANG Gui-ying2 1. Department of Oncologic Surgery,Affiliated Hospital of Hebei University,Baoding 071000,China,2. Department of Surgery,3. Department of Endoscopy,4. Department of Pathology,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011 |
| Abstract: | Objective: To explore the effect of filamin A (FLNa) gene on tumor formation activity of human colon carcinoma SW480 cells in nude mice and to elucidate the possible mechanism. Methods: Recombinant vector plasmid of pcDNA3.1/V5-His-TOPO/FLNa was transfected into SW480 cells by lipofectAMINE. The expression levels of FLNa mRNA and protein in SW480/FLNa cells were detected by RT-PCR and Western blotting. The proliferation rate of SW480/FLNa cells was determined by MTT assay. The SW480/FLNa, SW480/pcDNA3.1 and... |
| Keywords: | Colorectal neoplasms Mice nude Genes tumor suppressor Filamin A Cell SW480 |
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