核呼吸因子-1和Ki-67在乳腺癌组织中的表达及意义

探讨核呼吸因子-1（NRF-1）蛋白表达与乳腺癌发生发展、临床病理特征及Ki-67的关系。方法:采用免疫组化EnVision二步法，对211例乳腺癌组织、50例乳腺良性病变组织中NRF-1和Ki-67进行检测。结果:NRF-1表达定位于细胞核，在乳腺癌中表达率为87.2%，低于乳腺良性病变组织的100%（χ2=100.288，P<0.001）；高分化乳腺癌NRF-1表达率明显高于中、低分化乳腺癌（χ2=16.242，P=0.001；χ2=72.802，P<0.001），中分化乳腺癌也明显高于低分化乳腺癌（χ2=30.190，P=0.000）。乳腺癌淋巴结转移患者NRF-1表达率明显低于未转移者（χ2=12.025，P=0.007）；TNM分期中I期NRF-1表达率明显高于Ⅱ、Ⅲ期（χ2=12.025，P=0.007）。211例乳腺癌中Ki-67表达率为78.7%，Ki-67表达与NRF-1（χ2=42.914，P<0.001）、病理组织学分级（χ2=40.239，P<0.001）、淋巴结转移（χ2=16.061，P=0.001）和TNM分期（χ2=13.589，P=0.004）间比较差异有统计学意义。结论:NRF-1参与了乳腺癌的发生发展，也与Ki-67表达有相关性，可作为乳腺癌患者疾病进展监控和预后观测的指标，有可能成为乳腺癌新的治疗靶点。 

Expression of Nuclear Respiratory Factor-1 and Ki-67 in Breast Cancer and Its Significance

To investigate the correlation among the nuclear respiratory factor-1 ( NRF-1 ) expression and the carcinogenesis, invasion, metastasis, as well as Ki-67 expression, in breast cancer. Methods: NRF-1 and Ki-67 expression in 211 cases with breast cancer and 50 with benign lesion of the mammary gland were determined with immunohistochemical EnVision indirect method. Results: NRF-1 was located at the nucleus. The positive rates of NRF-1 expression were 82.7% in breast cancer and 100% in benign lesion of the mammary gland ( χ2 = 100.288, P = 0.000 ). NRF-1 expression rates were significantly higher in well-differentiated breast cancer than those in the moderately and poorly differentiated breast cancers ( χ2 = 16.242, P = 0.001; χ2 = 72.802, P = 0.000, respectively ). In addition, NRF-1 expression was higher in the moderately differentiated breast cancer than in the poorly differentiated breast cancer (χ2 = 30.190, P = 0.000 ). NRF-1 expression was lower in the patients with nodal metastasis than that in patients without a similar condition ( χ2 = 12.025, P = 0.007). The positive rate of NRF-1 was significantly lower in the TNM II and III stages than in the TNM I stage ( χ2 = 12.025, P = 0.007). The positive rate of Ki-67 was 78.7% in 211 patients with breast cancer. There were significant differences among the Ki-67 and NRF-1 expression rates ( χ2 = 42.914, P = 0.000), pathohistological grade ( χ2 = 40.239, P = 0.000), nodal metastasis ( χ2 = 16.061, P = 0.001), and TNM staging ( χ2 = 13.589, P = 0.004). Conclusion: NRF-1 expression plays an important role in the occurrence, as well as development, of breast cancer and correlates with Ki-67 expression. NRF-1 expression can be a marker in the progression and prognosis of breast cancer and may be used as the target for clinical treatment.