胆固醇酯转运蛋白对人宫颈癌HeLa细胞辐射增敏性的影响

目的:研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)高表达对宫颈癌HeLa细胞放射敏感性的影响。方法:采用脂质体介导法将构建插入全长CETP基因的pcDNA3载体转染人宫颈癌HeLa细胞,获得稳定高表达CETP的HeLa细胞,同时转染pcDNA3空质粒为对照;克隆形成实验检测细胞的存活率;FCM法检测细胞的凋亡率;蛋白质印迹法检测凋亡相关蛋白Bax、Bcl-2及核因子-κB(nuclear factor-kappa B,NF-κB)的表达。结果:X-射线照射后,CETP高表达的HeLa-CETP细胞的放射增敏比SERD0和SERDq分别为1.42和2.09,而未转染细胞与转染空质粒的细胞照射后,细胞存活率差异无统计学意义。FCM结果显示,在X-射线照射后,HeLa-CETP细胞的凋亡率明显高于未转染细胞与转染空质粒的细胞(P<0.05)。蛋白质印迹法检测结果显示,提高CETP的表达可以降低抗细胞凋亡相关蛋白Bcl-2和NF-кB的表达,而增加促细胞凋亡Bax蛋白的表达。结论:导入外源性CETP基因,提高HeLa细胞中CETP表达水平可明显增加HeLa细胞的放射敏感性,其作用机制可能与增加辐射诱导细胞凋亡和改变凋亡相关蛋白的表达有关。

Cholesteryl ester transfer protein enhances the radiosensitivity of human cervical cancer HeLa cells

Objective:To investigate the effect of cholesteryl ester transfer protein(CETP) on the radiosensitivity of human cervical cancer HeLa cells. Methods:HeLa-CETP cells with overexpression of CETP were established by stable transfection with pcDNA3 expression vector encoding a full-length of CETP gene. The HeLa cells which transfected with pcDNA3 empty vector were used as the control. The survival rate of HeLa-CETP cells was examined by clone formation assay. The apoptosis rate was detected by FCM. The expressi...