Identification of HIV-1 infected seropositive subjects by a direct diagnostic test involving hybridization of amplified viral DNA |
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Authors: | G Lucotte J Reveilleau |
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Affiliation: | Laboratoire d'Anthropologie Physique, Collège de France, Paris. |
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Abstract: | We describe a simple slot-blot hybridization procedure with an oligo-probe for identification of amplified HIV-1 DNA in seropositive subjects. Comparison are realized, on the same DNA samples, with other methods DNA detection including Southern blot tests after hybridization with a labelled probe, and autoradiographic patterns after digestion with a restriction enzyme of the amplified product hybridized. This new direct approach towards diagnosis of HIV-1 infection easily be carried out on a large scale. |
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