花刺参酸性粘多糖的分离研究

花刺参体壁经碱解、不同浓度的酒精沉淀氯化脱色及乙酸钾纯化,分别得花刺参酸性粘多糖SⅥ及SⅦ。2种多糖均显示电泳纯,超离心分析SⅥ S_(20w)=3.09,SⅦ S_(20w)=2.12;与碱性染料天青工异染反应,SⅥ和SⅦ的异染峰分别位移于514nm及507nm;SⅥ分子是由氨基半乳糖、葡萄糖醛酸、岩藻糖及硫酸基组成,其分子比值为1:1.21:1.29:4.62;SⅦ分子是由岩藻糖及硫酸基组成,其分子比值为1:0.99。而刺参粘多糖(Sjamp)的超离心分析S 20w=2.12。与碱性染料天青Ⅰ异染峰为515nm,Sjamp分子也是由氨基半乳糖,葡萄糖醛酸、岩藻糖及硫酸基组成,其分子比值为1:1.11:1.20:487。

STUDIES ON THE ISOLATION OF THE ACIDIC POLYSACCHARIDES IN STICHOPUS VARIEGATUS

After alkaine hydrolysis, the body wall of Slicliopus varicyatus was fractionally pre-cipitatecd with alcohol, then oxidatively decoloured and purified with KAc, the elcctrophorelically pure polysaccharides SVI and SVIIwere obtained. The results of ultracentrifugal analysis showed that the sedimentation coefficients were S20w 3. 09 for SVI and S20w 2. 12 for SVII. The metachromatic reaction with reddish black I showed the metachromatic peaks of SVI and SVII were shifted to 514nm and 507nm, respectively. The molecule of SVI is composed of aminogalactose, glucuronic acid, fucose and sulfate (1:1. 21:1. 29:4. 62) and that of SVII is composed of fucose and sulfate (1.0. 99). And our results of Stichopus japonicus acidic mucopolysaccharide ultracentrifugation therefore suggests that the sedimentation coefficients is S20w 2. 12. The metachromatic reaction with reddish black I showed the metachromatic peak is 515nm. The molecule of Sjamp is also composed of aminogalactose, glucuronic acid, fucose and sulfate ( 1:1. 1:1. 20:4. 87)